Fluorescent Proteins
2nd Edition
Published on 14. December 2007
660 pages
978-0-08-055724-3 (ISBN)
Description
This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
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Other editions
Additional editions
Kevin F. Sullivan
Fluorescent Proteins: Volume 85
Book
12/2007
2nd Edition
Academic Press
€163.69
Shipment within 15-20 days
Content
1. Fluorescent Proteins2. Functional Fusion Proteins by Random Transposon-Based GFP Insertion3. Fluorescent Proteins for Photoactivation Experiments4. Design and Optimization of Genetically Encoded Fluorescent Biosensors: Gtpase Biosensors5. Fast 4D Microscopy6. Single molecule imaging of fluorescent proteins7. Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins8. Fluorescent Protein Applications in Plants9. Expression and Imaging of Fluorescent Proteins in the C. elegans Gonad and Early Embryo10. Fluorescent Proteins in Zebrafish Cell and Developmental Biology11. Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain12. Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells13. Visualising mRNA Localisation and Local Protein Translation in Neurons14. Quantitative FRAP in Analysis of Molecular Binding Dynamics In Vivo 15. Quantitative and Qualitative Analysis of Plant Membrane Traffic Using Fluorescent Proteins16. Engineering FRET Constructs Using CFP and YFP17. Fluorescence Anisotropy Imaging Microscopy (FAIM) for homo-FRET in Living Cells18. FRET by Fluorescence Polarization Microscopy19. Bimolecular Fluorescence Complementation Visualization of Molecular Interactions in Living Cells20. Protein-protein interactions determined by fluorescence correlation spectroscopy21. Recent Advances on In Vivo Imaging with Fluorescent Proteins22. Computational Processing and Analysis of Dynamic Image Data23. Automated Classification of Mitotic Phenotypes of Human Cells Using Fluorescent Proteins24. Open Tools for Storage and Management of Quantitative Image Data
