Recombinant DNA And Cell Proliferation

ContributorsPrefaceI Expression of Specific Genes during the Cell Cycle 1 Use of Cloned SV40 DNA Fragments to Study Signals for Cell Proliferation I. Introduction II. Gene Transfer by Manual Microinjection III. SV40 and Cell Proliferation IV. A Growing Cell Receives Signals for Growth in Size V. A Growing Cell Receives Signals to Replicate DNA VI. Evidence That Signals for Growth in Size Can Be Separated from Signals for Cell DNA Replication VII. The Information for Growth in Size and for Cell DNA Replication Is Encoded in Different DNA Sequences of the SV40 A Gene VIII. Discussion References 2 Expression of Dihydrofolate Reductase and Thymidylate Synthase Genes in Mammalian Cells I. Overview II. Regulation of DHFR, TS, and TK Enzyme Levels in Normal Cells III. Isolation and Characterization of DHFR-Overproducing Cell Lines IV. DHFR Gene Expression in Overproducing Cells V. Isolation and Characterization of TS-Overproducing Cells References 3 Enzyme Expression during Growth and Cell Division in Saccharomyces cerevisiae: A Study of Galactose and Phosphorus Metabolism I. Introduction II. Regulation in the Galactose Metabolic Pathway III. Regulation of Phosphorus Metabolism IV. Concluding Remarks References 4 Gene Expression during the Cell Cycle of Chlamydomonas reinhardtii I. Introduction: Chlamydomonas as an Experimental Organism II. Characteristics of Synchronous Cultures of C. reinhardtii III. Expression of rRNA Genes during the Cell Cycle IV. Expression of Tubulin Genes during the Cell Cycle V. Ribulose-1,5-bisphosphate Carboxylase Synthesis during the Cell Cycle VI. Synthesis of Photosynthetic Membrane Proteins during the Cell Cycle VII. Conclusion References 5 Expression of Histone Genes during the Cell Cycle in Human Cells I. Introduction II. Structure and Organization of Human Histone Genes III. Levels of Control of Human Histone Gene Expression IV. Relationship between Histone Gene Expression and DNA Replication V. Conclusion References 6 Expression of the a-Fetoprotein Gene during Development, Regeneration, and Carcinogenesis I. Introduction II. Structure of the a-Fetoprotein Gene III. Methylation of the a-Fetoprotein Gene IV. Stability of a-Fetoprotein mRNA V. a-Fetoprotein mRNA during Development VI. a-Fetoprotein mRNA in Liver Regeneration and Neoplasia References 7 Organization and Expression of Eukaryotic Ribosomal Genes I. Introduction II. Isolation of Eukaryotic Ribosomal Protein Genes III. Characteristics of Eukaryotic Ribosomal Protein Genes IV. Metabolism of Ribosomal Protein V. Future Prospects ReferencesII Expression of Specific Genes Associated with Proliferation and Differentiation 8 Cell Cycle Dependence of Globin Gene Expression in Friend Cells I. Introduction II. Murine Erythroleukemia Cells (Friend Cells) III. Role of the Cell Cycle in Friend Cell Differentiation IV. Friend Cell Variants Temperature Sensitive for Growth V. Cell Cycle-Dependent Hemoglobin Synthesis in Temperature-Sensitive Friend Cells VI. Commitment: The Decision to Differentiate VII. Significance of the Cell Cycle during Commitment to Differentiation VIII. Conclusions and Future Prospects References 9 Proliferation, Differentiation, and Gene Regulation in Skeletal Muscle Myogenesis: Recombinant DNA Approaches I. Introduction II. Background III. Gene Regulation during Myogenesis IV. Control of Myogenesis: The Role of Mitogens V.