ICRF Handbook of Genome Analysis

The combined power of genetic analysis and recombinant DNAtechnology to analyse entire genomes has moved biomedical researchinto a new and revolutionary phase. The complete sequencing andmapping of the human genome, as well as the genomes of other modelorganisms, will be the basis for our future understanding of humandisease, and will allow us to answer fundamental questions aboutdevelopment and evolution. T The new ICRF Handbook of Genome Analysis is the essentialguide to the enormous range of techniques available to theresearcher for both the genetic and physical mapping of the genome,as well as the sequencing and analysis of DNA. It is both aprotocol manual and a comprehensive information resource. Writtenby international experts, each chapter presents a state-of-the-artreview of a methodology. Methods are fully described and evaluated;their advantages and disadvantages discussed; and their suitabilityfor different investigations considered. Step-by-step protocols,including computer analyses, are given for 123 essentialexperimental procedures. 'Troubleshooting' sections discusspossible reasons for failure and offer remedies. The primary focusis on human genetics and the benefits of an understanding of thegenome for the diagnosis and treatment of human disease. The bookalso considers the current state of progress in the analysis ofgenomes of many model organisms, including plants. A major part ofthe work provides detail on Internet resources as well as basicdata on human and other genomes, including mapped disease genes andmouse knockouts. * Covers not only the human genome in relation to cancers andother human diseases, but also the genomes of all important modelorganisms * Contains 123 easy-to-follow protocols for essentialexperimental procedures * Reviews a vast range of other information resources, includingjournals and the Internet * provides an invaluable listing ofsuppliers of laboratory materials * Has been written by international experts from their ownpractical experience * Is mandated by the Imperial Cancer Research Fund - a leader inresearch in this field * Has a sturdy spiral binding within a hardback case for ease ofuse in the lab