Animal Cell Technology

Section 1. Chairman's Statement Presence and Future of Animal Cell Technology Section 2. Making and Selecting Recombinant Cell Lines Introduction of DNA Into Mammalian Cells Retrotargeting: Use of Defective Retroviral DNA Fragments to Improve Recombinant Protein Production in Mammalian Cells Receptor-Mediated Gene Delivery into Mammalian Cells Improvement of Cell Lines for Large Scale Mammalian Cell Culturing Drosophila Cell Lines as Hosts for Recombinant Protein Expression Immortalized Cell Lines for Virus Diagnosis Selection Strategies for Highly Productive Recombinant Cell Lines Section 3. Cell Line Characterization Population Analysis of a Recombinant Chinese Hamster Ovary Cell Line Expressing Recombinant Human Protein Cultured in the Presence and Absence of Methotrexate Selective Pressure Genetic and Biochemical Analysis of a Murine Hybridoma in Long-Term Continuous Culture DNA Fingerprinting as a Quality Control Marker for the Genetic Stability of Production Cells Analysis of SV40 Early Region Expression in Immortalized Mouse Macrophage Cell LinesSection 4. Complex Media formulations Stimulation of Murine Hybridomas by Cytokines The Use of 2-Hydroxy-2,4,6-Cycloheptarin-1-One (Tropolone) as a Replacemetn for Transferrin Comparison of Long R3IGF-1 with Insulin in the Support of Cell Growth and Recombinant Protein Expression in CHO Cells Development of a Hybridoma Cloning Medium Performance of Gamma-Irradiated Fetal Bovine Serum in Cell Culture Low Level FCS Adaptation and Media Development for the Culture of Two Hybridoma Cell Lines Producing Igg and IGM Monoclonal Antibodies Improved Bioreactor Productivity and Manufacturing Efficiency Using Liquid Medium Concentrates Cell Culture Raw Materials Screening by Calcein-AM Fluorescence Using a 96 Well Plate format Importance of Extra-Cellular Matrix in the Long Term Anchorage Dependent Growth of Cell Line CHO K1Section 5. Protein and or Serum Free Cultures Development of Serum Free Media for Engineered NSO Cell Lines Chloresterol: An Important Additive for Serum-Free Media? Serum Free Ultraculture? Medium: An Alternative to the Instability Generated by Long-Term Cultivation of Mouse Hybridomas Investigations of the TSH Antibody Producing Mouse Hybridoma Cell Line under Serum-Free Media Condition Interferon Gamma Production in Laboratory Bioreactor by Lens Culinaris Lectin Stimulated Human Buffy Coat Using Protein Free Medium Evaluation of CHO Cell Growth in a Protein-Free Environment Inactivation of Serum Contaminants Adaption of Mammalian Cells to Protein-Free Growth Section 6. Ammonia/Glutamine and other Inhibitors Ammonium Ions Inhibit Cell Growth by Intracellular formation of UDP-Activated Hexosamines Influence of the PH on the Ammonia Sensitivity of a Murine Hybridoma Cell Line The Influence of Ammonium on T-Pa Production from Chinese Hamster Ovary (CHO) Cells Detoxification of Ammonium in Bioreactor Cultivations Influence of Lactate and Ammonia on the Death Rate of Hybridoma The Effect of Glutamine-Containing Dipeptides on the Growth and Productivity of a Hybridoma Inhibitory Substance(S) Secreted in Cell Culture Media of Recombinant CHO and a Hybridoma Cell Line Growth of Hybridoma Cells is Inhibited by GangliosidesSection 7. Apoptosis and Cell Biology Programmed to Die: Cell Death via Apoptosis Cell Death (Apoptosis) in Cell Culture Cell Death by Necrosis and Adoptosis during the Culture of Commercially Important Cell Lines Spontaneous Apoptosis Contributes Negatively to the Regulation of Hybridoma Cell Viability Metabolic Characteristics and Specific Requests of Hybridoma and CHO Cell Lines Nature of Cell-Cell Interactions Between Recombinant CHO Cells Expressing Human Interferon GammaSection 8.