Animal Cell Technology
Developments, Processes and Products
Published on 2. October 2013
784 pages
978-1-4831-4507-5 (ISBN)
Description
Animal Cell Technology: Developments, Processes and Products is a compilation of scientific papers presented at the 11th European Society for Animal Cell Technology (ESACT) Meeting, held in Brighton, United Kingdom. The book is a collection of various works of scientists, engineers, and other specialists from Europe and other parts of the world who are working with animal cells. The book's aim is to communicate experiences and research findings on the development of cell systems. The research papers are grouped into 25 sections encompassing 145 chapters. Subjects covered range from cells and physiology engineering dealing with cell characterization, cell culture establishment, cloning, and cell engineering. Topics on culture media, ammonium detoxification, the effects of physical parameters on cell cultures, assays and monitoring systems, and bioreactor techniques are also covered. Discussions are likewise made on the products from animal cells in culture, virus removal, and DNA determination and characterization in relation to safety issues. The book will be useful for cell biologists, molecular biologists, biochemists, biochemical engineers, and students engaged in the study of animal cell cultures.
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06/1992
Butterworth-Heinemann
€123.80
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Content
Section 1. Cell Characterization The Characterization of SV40-Immortalized Murine Macrophage Cell Lines Identification of Type-D Retroviruses as Contaminants in Widely Used Cell Lines Characterization of Human Endothelial Hybrid Cells Expressing Von Willebrand Factor Primary Cultures of Human Proximal Tubule Cells (HPTC) in Serum Free Defined Media: A Characterization Study FSection 2. Cell Culture Establishment Culture of Cells from Adult Zebrafish (Brachydanio Rerio) and Embryo Cloning of Mouse Hybridoma Cells Infected With MYC and RAS-Containing Retroviruses Yields Cell Lines with Improved Growth and Antibody Production Survival of B Lymphocytes Is Prolonged after Infection with Immortalizing Viruses Establishment of Dermal Papilla Cell Lines by Retrovirus-Mediated Transfer of Oncogenes Immortalization of Flatfish (Paralichthys Olivaceus) Leukocytes by Oncogene TransfectionSection 3. Engineering Cells for Expression Host Cell Derived Retroviral Sequences Enhance Transfection and Expression Efficiency in CHO Cells Stability of Production of Recombinant Antibodies from Glutamine Synthetase Amplified CHO and NSO Cell Lines The Rapid Production of Recombinant Rabbit Metalloproteinases in Myeloma Cells Long Term Stability of Expression of Humanized Monoclonal Antibody Campath 1-H in Chinese Hamster Ovary Cells Oncogene-Activated Production of Recombinant Proteins for Animal Cells Immunoglobulin Gene Expression during Hybridoma Batch and Semicontinuous Cultures A Novel Mammalian Expression System Based on the Globin Locus Control Region Transient Increase of Production in Fused Recombinant CHO Cells A Comparison of Eukaryotic Control Elements Using Tissue Plasminogen Activator (tPA) as a Reporter GeneSection 4. Physiological Modulation of Expression Dissociation of MAB Production from Cell Division Using DNA Biosynthesis Inhibitors The Effect of Tetramethyl Urea ¿-Interferon Production by Namalwa Cells The Effect of Sequential Induction of A-Interferon Production by Namalwa Cells The Influence of Some Natural and Synthetical Substrata upon the Behavior of Some Cell Lines In Vitro Differentiated Liver Function in Rat Hepatocytes Transfected with SV40 Viral DNA Determinant Factors for Expression of Recombinant Genes in CHO Cells New Kinetic Parameters of the Growth of Animal CellsSection 5. Culture Media The Preparation from Human Plasma of a Cell Medium Supplement than Can Be Pasteurized Free Fatty Acid Utilization of Different Hybridoma Cell Lines and Its Effect on Cell Growth, Metabolism and Antibody Formation Pluronic Adsorption Effects in Cell Culture Media Influence of Medium Composition on the Death and Lysis of Hybridoma Cells in Continuous CulturesSection 6. Serum Free Media Basic Structure of a Databank - Knowledge Based System for the Computer Aided Design of Serum-Free Cell Culture Media Long Term Serum-Free Hollow-Fiber Culture of Cell Lines Producing Human Monoclonal Antibodies: Metabolic Aspects Serum Free Cryopreservation of Antibody Producing Cells Growth of HeLa Cells in a Chemically Defined Medi-Cult® Medium Adaptation of rCHO Cells to Growth in Serum Free Medium: Flow Cytometric Study Growth, Subculture and Cryopreservation of Anchorage-Dependent Cells: A Complete Serum-Free System Cryoprotection and Cultivation of Hybridoma Cell Lines under Serum Free Condition Production and Characterization of a Recombinant Factor VIII Expressed by CHO Cells Cultured in Serum Supplemented or in Serum Free Media A Defined Serum-Free Medium for Diverse Cell Culture Applications Adaptation of a Murine Monoclonal Hybridoma Cell Line to a Low Cost Serum-Free Medium by a Repeated Batch Fermentation ProcedureSection 7.
