Alternative Splicing
Methods and Protocols
Published on 27. July 2022
XI, 354 pages
978-1-0716-2521-7 (ISBN)
Description
This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful
Methods in Molecular Biology
series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
More details
Series
Language
English
Place of publication
New York
United States
Publishing group
Springer US
Target group
Professional and scholarly
Product notice
Reflowable
Illustrations
XI, 354 p. 61 illus., 57 illus. in color.
File size
10,58 MB
ISBN-13
978-1-0716-2521-7 (9781071625217)
DOI
10.1007/978-1-0716-2521-7
Schweitzer Classification
Other editions
Additional editions
Book
07/2022
Humana
€235.39
Shipment within 15-20 days
Content
Alternative Splicing in Human Biology and Disease.- Targeting Alternative Splicing for Therapeutic Interventions.- An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions.- FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis.- Quantitative Measurement of Alternatively Spliced RNA Isoform Levels.- Analysis of Splicing Regulation by Third-Generation Sequencing.- Computational Analysis of Alternative Splicing Using
VAST-TOOLS
and the
VastDB
Framework.- Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data.- Functional Annotation of Custom Transcriptomes.- Design, Labeling, and Application of Probes for RNA smFISH.- BaseScopeT Approach to Visualize Alternative Splice Variants in Tissue.- In Situ Imaging of mRNA Splicing Variants by SpliceRCA.- Two-Color Fluorescent Reporters for Analysis of Alternative Splicing.- Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry.- Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy.- Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention.- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins.- Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo.- Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.
