Vectors
A Survey of Molecular Cloning Vectors and Their Uses
Published on 20. May 2014
592 pages
978-1-4831-0308-2 (ISBN)
Description
Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.
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12/1987
Butterworth-Heinemann
€86.66
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Content
PrefacePart I. Bacterial Cloning Vectors General Cloning Vectors for Gram-Negative Bacteria 1. The Plasmid, pBR322 2. Bacteriophage Lamba Cloning Vectors 3. Filamentous Phages as Cloning Vectors 4. Chimeric Single-Stranded DNA Phage-Plasmid Cloning Vectors 5. Phage-Plasmid Hybrid Vectors 6. Cosmids Specialized Cloning Vectors for Gram-Negative Bacteria 7. Plasmid Positive Selection Vectors 8. Vectors for Cloning Promoters and Terminators 9. A Survey of Vectors for Regulating Expression of Cloned DNA in E. Coli 10. Expression Vectors Employing Lambda-, trp-, lac-, and lpp-Derived Promoters 11. Vectors with Adjustable Copy Numbers 12. Specialized Plasmid Vectors for Cloning cDNA 13. Vectors for the Synthesis of Specific RNAs In Vitro 14. Improved Suppressor tRNA Cloning Vectors and Plasmid - Phage Recombination Cloning Vectors with Broad-Host-Range Capability 15. Broad-Host-Range Plasmid Cloning Vectors for Gram-Negative Bacteria 16. Specialized Vectors for Members of Rhizobiaceae and Other Gram-Negative Bacteria Cloning Vectors for Gram-Positive Bacteria 17. Generalized Cloning Vectors for Bacillus Subtilis 18. Promoter Probe Plasmids for Gram-Positive BacteriaPart II. Fungal Cloning Vectors 19. Molecular Cloning Vectors of Saccharomyces: Generalized Cloning Vectors 20. Plasmid Vectors for the Analysis of Regulatory Sequences in Yeast 21. Molecular Cloning Vectors for Aspergillus and NeurosporaPart III. Insect and Animal Cell Cloning Vectors 22. Vectors for P-Mediated Transformation in Drosophila 23. Baculoviruses for Foreign Gene Expression in Insect Cells 24. Mammalian Expression Vectors 25. Retroviral VectorsPart IV. Plant Cloning Vectors 26. Agrobacterium tumefaciens Ti Plasmid-Derived Plant Vectors for Dicotyledonous and Monocotyledonous Plants 27. Current Vectors for Plant TransformationIndex
