Regulators and Effectors of Small GTPases: Ras Family

Real-time in vitro measurement of Intrinsic and Ras GAP-mediated GTP hydrolysis; Schwann cell preparation from single mouse embryos: Analyses of neurofibromin function in Schwann cells; Regulation of the nucleotide state of oncogenic Ras proteins by nucleoside diphosphate kinase; Measurements of TSC2 GAP activity toward Rheb; Characterization of AND-34 function and signalling; Studying the spatial and temporal regulation of Ras GTPase-activating proteins; Activation of Ras Proteins by Ras Guanine Nucleotide Releasing Protein Family Members; Ras and Rap1 Activation of PLC? lipase activity; Specificity and expression of RalGPS as RalGEFs; Biochemical and biological analyses of Rgr RalGEF oncogene; Analysis of Ras Activation in Living Cells with GFP-RBD; Genetic and pharmacologic analyses of the role of Icmt in Ras membrane association and function; Characterization of the activation of the Rap specific exchange factor epac by cyclic nucleotides; Biochemistry of the Rap-specific guanine nucleotide exchange factors PDZ-GEF1 and 2; Characterization of interactions between Ras family GTPases and their effectors; Genetic and pharmacologic dissection of Ras effector utilization in oncogenesis; Sequencing analysis of BRAF mutations in human cancers; KSR regulation of the Raf-MEK-ERK cascade; Ras-sensitive IMP modulation of the Raf/MEK/ERK cascade through KSR1; Raf Kinase Inhibitor Protein (RKIP) regulation of Raf and MAPK signalling; Harnessing RNAi for Analyses of Ras Signaling and Transformation; The Rac Activator Tiam1and Ras induced Oncogenesis; Phospholipase Ce CfÕ guanine nucleotide exchange factor activity and activation of Rap1; Nore1 and RASSF1 regulation of cell proliferation and of the MST1/2 kinases; RASSF family proteins and Ras transformation; Ras and the Rain/RasBP1 effector; The RIN family of Ras effectors; RAP1 regulation of RIAM and cell adhesion; Regulation of cell-cell adhesion by Rap1; Effects of Ras signaling on gene expression analyzed by customized microrays; Protein-fragment complementation assays (PCA) in small GTPase research and drug discovery; Ras Up-regulation of Cycloxygenase-2; Regulation of the expression of tropomyosins and actin cytoskeleton by ras-transformation; Regulation of Par-4 by Oncogenic Ras; Using Drosophila and yeast genetics to investigate a role for the RhebGTPase in cell growth; Biochemistry and Biology of ARHI (DIRAS3), An Imprinted Tumor Suppressor Gene Whose Expression Is Lost In Ovarian and Breast Cancer; Gem protein signaling and regulation; Analysis of Rem/RGK Signaling and Biological Activity; Analysis of Rit Signaling and Biological Activity; Characterization of RERG: An estrogen-regulated tumor suppressor gene; Inhibition of transcription factor NF-?B activation by ?B-Ras; Analysis of Rhes activation state and effector function ; Rheb activation of mTOR and S6K1 signaling; Use of Retrovirus Expression of Interfering RNA to Determine the Contribution of Activated K-Ras and Ras Effector Expression to Human Tumor Cell Growth; Using inhibitors of prenylation to block localization and transforming activity; Sorafenib (BAY 43-9006), a dual action inhibitor that targets RAF/MEK/ERK pathway in tumor cells and tyrosine kinases VEGFR/PDGFR in tumor vasculature; Yeast screens for inhibitors of Ras-Raf interaction and characterization of MCP inhibitors of Ras-Raf interaction; A tagging-via-substrate technology for genome-wide detection and identification of farnesylated proteins; A genetically defined normal human somatic cell system to study Ras oncogenesis in vivo and in vitro; Analyses of Ras transformation of human thyroid epithelial cells; Use of Ras-transformed human ovarian surface epithelial cells as a model for studying ovarian cancer; Physiological analysis of oncogenesic Kras; Use of conditionally active Ras Fusion Proteins to study Epidermal Growth, Differentiation and Neoplasia; Pancreatic duct epithelial cell isolation and cultivation in two dimensional and three dimensional