Regulators and Effectors of Small GTPases, Part F: Ras Family I

Name: Regulators and Effectors of Small GTPases, Part F: Ras Family I | Ras Family I
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Ras Family I

Academic Press

Published on 17. April 2001

492 pages

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978-0-08-052255-5 (ISBN)

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Front Cover
Regulators and Effectors of Small GTPases
Copyright Page
Table of Contents
Contributors to Volume 332
Preface
Volumes in Series
Section I: Protein Expression and Protein-Protein Interactions
Chapter 1. Mammalian Expression Vectors for Ras Family Proteins: Generation and Use of Expression Constructs to Analyze Ras Family Function
Chapter 2. Protein Transduction: Delivery of Tat-GTPase Fusion Proteins into Mammalian Cells
Chapter 3. Green Fluorescent Protein-Tagged Ras Proteins for Intracellular Localization
Chapter 4. Targeting Proteins to Membranes, Using Signal Sequences for Lipid Modifications
Chapter 5. Targeting Proteins to Specific Cellular Compartments to Optimize Physiological Activity
Chapter 6. Mapping Protein-Protein Interactions with Alka- line Phosphatase Fusion Proteins
Chapter 7. Assays of Human Postprenylation Processing En- zymes
Chapter 8. In Vivo Prenylation Analysis of Ras and Rho Pro- teins
Chapter 9. Ras Interaction with RalGDS Effector Targets
Chapter 10. RAS Interaction with RIN1 Effector Target
Chapter 11. Ras and Rap1 Interaction with AF-6 Effector Target
Section II: Screening Analyses
Chapter 12. Analysis of Protein Kinase Specificity by Peptide Libraries and Prediction of in Vivo Substrates
Chapter 13. Peptide Library Screening for Determination of SH2 or Phosphotyrosine-Binding Domain Sequences
Chapter 14. Expression Cloning of Farnesylated Proteins
Chapter 15. Expression Cloning to Identify Monomeric GTP- Binding Proteins by GTP Overlay
Chapter 16. Retrovirus cDNA Expression Library Screening for Oncogenes
Chapter 17. Identification of Ras-Regulated Genes by Representational Difference Analysis
Chapter 18. Differential Display Analysis of Gene Expression Altered by ras Oncogene
Chapter 19. cDNA Array Analyses of K-Ras-Induced Gene Transcription
Chapter 20. Ras Signaling Pathway for Analysis of Protein-Protein Interactions
Chapter 21. Isolation of Effector-Selective Ras Mutants by Yeast Two-Hybrid Screening
Chapter 22. Two-Hybrid Dual Bait System to Discriminate Specificity of Protein Interactions in Small GTPases
Chapter 23. Functional Proteomics Analysis of GTPase Signal- ing Networks
Section III: Analyses of Mitogen-Activated Protein Kinase Cascades
Chapter 24. Analyzing JNK and p38 Mitogen-Activated Protein Kinase Activity
Chapter 25. Phospho-Specific Mitogen-Activated Protein Kinase Antibodies for ERK, JNK, and p38 Activation
Chapter 26. Immunostaining for Activated Extracellular Signal-Regulated Kinases in Cells and Tissues
Chapter 27. Dominant Negative Mutants of Mitogen-Activated Protein Kinase Pathway
Chapter 28. Scaffold Protein Regulation of Mitogen-Activated Protein Kinase Cascade
Chapter 29. Bacterial Expression of Activated Mitogen-Activated Protein Kinases
Chapter 30. Steroid Receptor Fusion Proteins for Conditional Activation of Raf-MEK-ERK Signaling Pathway
Chapter 31. Pharmacologic Inhibitors of MKK1 and MKK2
Chapter 32. Analysis of Pharmacologic Inhibitors of Jun N-Ter- minal Kinases
Author Index
Subject Index

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Regulators and Effectors of Small GTPases, Part F: Ras Family I

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