RNA-Ligand Interactions, Part B: Molecular Biology Methods
Molecular Biology Methods
Academic Press
Published on 26. June 2000
539 pages
978-0-08-049675-7 (ISBN)
Description
RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods.RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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Daniel W. Celander | John N. Abelson
RNA-Ligand Interactions, Part B: Molecular Biology Methods: Volume 318
Book
06/2000
Academic Press
€167.13
Withdrawn from sale
Persons
Content
- Front Cover
- Rna-Ligand Interactions
- Copyright Page
- Table of Contents
- Contributors to Volume 318
- Preface
- Volumes in Series
- Section I: Solution Probe Methods
- Chapter 1. Probing RNA Structure and RNA-Ligand Complexes with Chemical Probes
- Chapter 2. Methylene Blue-Mediated Cross-Linking of Proteins to Double-Stranded RNA
- Chapter 3. Structure and Distance Determination in RNA with Copper Phenanthroline Probing
- Chapter 4. Applications of Uranyl Cleavage Mapping of RNA Structure
- Chapter 5. Identification of Messenger RNAs That Contain Inosine
- Section II: Tethered-Probe Methodologies
- A. Photochemical Reagents
- Chapter 6. Site-Specific 4-Thiouridine Incorporation into RNA Molecules
- Chapter 7. Nucleoprotein Photo-Cross-Linking Using Halo- pyrimidine-Substituted RNAs
- Chapter 8. Structure Determination by Directed Photo-Cross- Linking in Large RNA Molecules with Site-Specific Psoralen
- Chapter 9. Photolabile Derivatives of Oligonucleotides as Probes of Ribosomal Structure
- Chapter 10. Photoaffinity Cross-Linking and RNA Structure Analysis
- B. Chemical Reagents
- Chapter 11. Cleavage of RNA with Synthetic Ribonuclease Mimics
- Chapter 12. Site-Specific Sulfhydryl Groups for Study of RNA Conformation via Disulfide Cross-Linking
- Chapter 13. Directed Hydroxyl Radical Probing Using Iron(II) Tethered to RNA
- Section III: In Vitro Affinity Selection Methodologies
- Chapter 14. In Vitro Selection of RNA Aptamers
- Chapter 15. In Vitro Selection and Characterization of RNAs with High Affinity to Antibiotics
- Chapter 16. Selection for RNA: Peptide Recognition through Sulfur Alkylation Chemistry
- Chapter 17. In Vitro Selection of RNA Substrates for Ribonuclease P and Its Catalytic RNA
- Chapter 18. RNA-Protein Interactions in Ribosomes. In Vitro Selection from Randomly Fragmented rRNA
- Chapter 19. Optimized Synthesis of RNA-Protein Fusions for in Vitro Protein Selection
- Section IV: Genetic Methodologies for Detecting RNA-Protein Interactions
- A. Bacterial Systems
- Chapter 20. Screening RNA-Binding Libraries by Transcriptional Antitermination in Bacteria
- Chapter 21. Rapid Genetic Analysis of RNA-Protein Interactions by Translational Repression in Escherichia coli
- Chapter 22. RNA Challenge Phages as Genetic Tools for Study of RNA-Ligand Interactions
- B. Eukaryotic Systems
- Chapter 23. Screening RNA-Binding Libraries Using Tat-Fusion System in Mammalian Cells
- Chapter 24. Frameshifting Assay to Characterize RNA-Protein Interactions in Eukaryotic Cells
- Chapter 25. Translational Repression Assay Procedure. A Method to Study RNA-Protein Interactions in Yeast
- Chapter 26. Analysis of Low-Abundance Ribonucleoprotein Particles from Yeast by Affinity Chromatography and Mass Spectrometry Microsequencing
- Chapter 27. Yeast Three-Hybrid System to Detect and Analyze RNA-Protein Interactions
- Section V: Protein Engineering Methodologies Useful for RNA-Protein Interaction Studies
- Chapter 28. Using Peptides as Models of RNA-Protein Interactions
- Chapter 29. Analysis of RNA-Protein Cross-Link Sites by BERND THIEDE AND Matrix-Assisted Laser Desorption/Ionization BRIGITTE WITTMANN-LIEBOLD Mass Spectrometry and N-Terminal Microsequencing
- Chapter 30. In Vitro Reconstitution of 30S Ribosomal Subunits Using Complete Set of Recombinant Proteins
- Chapter 31. Directed Hydroxyl Radical Probing of RNA from Iron(II) Tethered to Proteins in Ribonucleoprotein Complexes
- Section VI: Cell Biology Methods
- Chapter 32. Use of Dimethyl Sulfate to Probe RNA Structure in Vivo
- Chapter 33. Sensitive and High-Resolution Detection of RNA in Situ
- Author Index
- Subject Index
- Color Plate Section
