Protein Termini Part B
Elsevier (Publisher)
Published on 25. September 2025
258 pages
978-0-443-47193-3 (ISBN)
Description
Protein termini represent a major route to protein regulation. From the moment the very first amino acid of a polypeptide chain exits the ribosome there is potential for steering from the cellular environment. This volume of Methods in Enzymology Modifications and Targeting of Protein Termini focuses on Protein N-termini and C-termini and their modifications which include acetylation, arginylation, myristoylation and oxidation. Also, the impact of terminal modifications is covered, in particular the impact on protein turnover and the ubiquitin E3 ligases which specifically recognize protein N-termini (N-degrons) and C-termini (C-degrons). In addition to the detailed methods and laboratory protocols, the chapters include informative overviews and reviews of the different subfields.
- Provides the authority and expertise of leading contributors from an international board of authors
- Includes the latest information on modifications and targeting of proteins via their N- and C-terminal ends
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Thomas Arnesen
Protein Termini Part B: Volume 719
Book
09/2025
Academic Press
€170.84
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Persons
Content
1. Proteome analysis of puromycin-labeled nascent polypeptidesKoshi Imami2. HUNTER-DIA: An updated protocol for enrichment and mass spectrometry-based identification of protein N-terminiPitter F. Huesgen3. TERMINER - Bioinformatic detection and annotation of proteolytic protein termini in shotgun proteomics dataOliver Schilling4. Degronopedia: A practical guide to identifying and targeting protein degronsWojciech Pokrzywa5. Quantitative Insights into Protein Turnover and Ubiquitination with HiBiT and NanoBRETWojciech Pokrzywa6. Development of a flow cytometric method to evaluate the impact of N-terminal sequences on protein stabilityAditya M. Kunjapur7. Generation and characterization of engineered N-degrons of the N-degron pathway using the ubiquitin-reference techniqueChang Hoon Ji8. Characterization of the autophagic N-degron pathway and monitoring its chemical modulation for therapeutic developmentYong Tae Kwon9. Characterization of the E3 ligase KCMF1 as a ZZ/N-recognin of the autophagic Arg/N-degron pathwayChang Hoon Ji10. Identification of Ac/N-degron-recognition domain within the MARCHF6 E3 ubiquitin ligaseCheol-Sang Hwang11. Affinity purification-mass spectrometry to identify nuclear protein interactions of N-terminal acetyltransferase NAA40Antonis Kirmizis12. TurboID technique for proximity labelling of interacting proteinsGreta Jarck13. Chemical proteomic approaches to investigate S-prenylationEdward W. Tate14. Quantitative analysis of C-terminal prenylated protein levels using tandem mass taggingMark Distefano15. Optimizing purification and FP-based binding assays for the E3 ligase FEM1CRong Huang
