
Progress in Nucleic Acid Research and Molecular Biology
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Content
- Cover
- Contents
- List of Contributors
- Preface
- Abbreviations and Symbols
- Contents of Volume 1
- Contents of Volume 2
- Contents of Volume 3
- Articles Planned for Future Volumes
- Chapter 1. Isolation and Fractionation of Nucleic Acids
- I. Introduction
- II. Isolation of Deoxyribonucleic Acids
- III. Separation of DNA from RNA
- IV. Purification of DNA
- V. Fractionation of DNA
- VI. Soluble or Transfer RNA (sRNA)
- VII. Ribosomal RNA
- VIII. Virus RNA
- IX. Purification of RNA
- X. Fractionation of RNA
- XI. Messenger RNA (Informational RNA)
- XII. Conclusion
- References
- Chapter 2. Cellular Sites of RNA Synthesis
- I. Introduction
- II. Time Course of RNA Labeling in Intact Cells
- III. RNA Migration and Nuclear Transplantation
- IV. Intracellular Transfer of RNA during Mitosis
- V. RNA Synthesis and Mitosis
- VI. Enucleation of Cells and RNA Synthesis
- VII. RNA Synthesis and Erythrocyte Formation
- VIII. RNA Synthesis during DNA Synthesis
- IX. Actinomycin D Inhibition of RNA Synthesis
- X. Nucleolar Origin of Ribosomal RNA
- XI. Site of sRNA Synthesis
- XII. Intranuclear Shifts of RNA
- XIII. Conclusion
- References
- Chapter 3. Ribonucleases in Taka-Diastase: Properties, Chemical Nature, and Applications
- I. Introduction
- II. Nomenclature
- III. Preparation and Properties
- IV. Structure and Function
- V. Specificity
- VI. Applications
- References
- Chapter 4. Chemical Effects of Ionizing Radiations on Nucleic Acids and Related Compounds
- I. Physicochemical Basis
- II. Pyrimidines
- III. Purines
- IV. Nucleosides and Nucleotides
- V. Nucleic Acids
- VI. Nucleoproteins
- References
- Chapter 5. The Regulation of RNA Synthesis in Bacteria
- I. Introduction
- II. Regulation of rRNA Synthesis
- III. Regulation of sRNA Synthesis
- IV. Regulation of mRNA Synthesis
- V. A New Strategy for Studying Regulation
- VI. Summary
- Addendum
- References
- Chapter 6. Actinomycin and Nucleic Acid Function
- I. Introduction
- II. Molecular Basis of Action of Actinomycin
- III. The Use of Actinomycin in the Study of Gene Action
- IV. Summary
- References
- Chapter 7. De Novo Protein Synthesis in Vitro
- I. Introduction
- II. Definition of de Novo Synthesis
- III. Mechanism of Formation of Polypeptide Chains
- IV. Specific Protein Synthesis
- V. Cell-Free Induction and Repression in Membrane Fractions from E. coli
- VI. Biosynthesis of Alkaline Phosphate by Enzyme-Forming Particles (EFP) of E. coli
- VII. Concluding Remarks Referencw
- References
- Chapter 8. Free Nucleotides in Animal Tissues
- I. Introduction
- II. History
- III. The Metabolic Roles of Various Nucleotides
- IV. Methods of Separation and Identification of Free Nucleotides
- V. Distribution of Free Nucleotides in Tissues
- VI. Changes in Free Nucleotide Pools in Various Physiological and Experimental Conditions
- VII. Discussion and Conclusions
- References
- Index
- Author Index
- Subject Index
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