Cell Separation

PrefaceContents of Previous Volumes1. Isolation and Characterization of Human Vascular Endothelial Cells with Application to Studies of the Subendothelial Matrix I. Introduction II. Isolation of Endothelial Cells III. Isolation and Characterization of the Subendothelial Matrix IV. Concluding Remarks References2. Separation and Characterization of Lymphoid Cells from Mouse Mammary Carcinomas by Isokinetic Sedimentation I. Introduction II. Separation of Lymphoid Cells from Mouse Mammary Tumors III. Comparison of Results with Isokinetic Sedimentation and Centrifugal Elutriation IV. Conclusion References3. Separation of Malignant Cells from Host Cells Using Centrifugal Elutriation I. Introduction II. Theoretical and Practical Aspects III. Isolation of Host Cells from Tumor Cells IV. Isolation of Cell Subpopulations from Neoplastic Cells V. Conclusions References4. Selection of Tumor Cell Metastatic Variants by Differential Adhesion to Immobilized Lectins I. Introduction II. Experimental Methods III. Fractionation of Murine Large-Cell Lymphoma Cells IV. Discussion of the Technique References5. Purification of Antigen-Specific ¿ Cells by Adherence to Whole-Cell Antigens I. Introduction II. Methods III. Assessment of the Technique IV. Comments on the Technique References6. Separation and Characterization of Subpopulations of ¿ Cells I. Introduction II. Physiochemical Characteristics of ¿ Lymphocytes III. Surface Markers of ¿ Lymphocytes IV. Separation of ¿ Lymphocyte Subpopulations V. Concluding Remarks References7. Separation of Pancreatic Islet Cells according to Functional Characteristics I. Introduction II. Isolation of Pancreatic Islets III. Preparation of Islet Cells IV. Autofluorescence-Activated Sorting of Islet Cells V. Applicability to Other Cells VI. Summary References8. Separation and Characterization of Leukocytes from the Intestine I. Introduction II. Distribution of Lymphoid Cells within the Intestine III. Isolation of Cells from Peyer's Patches IV. Isolation of Intraepithelial Leukocytes (IEL) V. Isolation of Cells from the Lamina Propria VI. Problems Associated with Isolation VII. Properties of Intestinal Leukocytes VIII. Concluding Remarks References9. Separation of Epithelial Cells from Gastric and Intestinal Mucosa I. Introduction II. Techniques for Isolating Gastric and Intestinal Mucosal Cells III. Techniques for Cell Separation IV. Culture of Dispersed Gastrointestinal Epithelial Cells V. Morphology of Isolated Cells VI. Functional Characterization of Isolated Gastrointestinal Cells VII. Concluding Remarks References10. Separation of Cells from Normal and Abnormal Epidermis I. Introduction II. Methods of Epidermal Separation III. Methods of Epidermal Cell Separation IV. Biology and Pathobiology of Epidermal Cell Subpopulations V. Conclusion References11. Isokinetic Gradient Sedimentation of Hematopoietic Progenitor Cells I. Introduction II. Preparation of Bone Marrow Cells and Gradients III. Isokinetic Gradient Sedimentation of Mouse Bone Marrow Cells IV. Isokinetic Gradient Sedimentation of Human Bone Marrow Cells V. Concluding Remarks References12. Purification of Osteoblasts I. Introduction II. Separation of Osteoblasts III. Purification Methods IV. Problems of Separated Cells V. Identification of Osteoblast VI. Purification of Osteoblasts by FACS VII. Applications References13. Isolation and Separation of Human Colostra! and Milk Cells I. Introduction II. Whole-Cell Populations III. Adherence IV. Isopycnic Centrifugation V. Velocity Sedimentation and Isokinetic Centrifugation VI. Evaluation of Various Techniques References14.