Cell Separation

Preface1. Separation and Characterization of Liver Cells I. Introduction II. Characterization of Liver Cells III. Preparation of Isolated Liver Cells IV. Conclusions References2. Purification and Characterization of Epidermal Langerhans Cells I. Introduction II. Cellular Constituents of the Epidermis III. Characterization of Epidermal Langerhans Cells IV. Enrichment of Epidermal Langerhans Cells References3. Purification and Culture of Oval Cells from Rat Liver I. Biological Importance of Oval Cells: Does Normal Adult Liver Contain Stem Cells? II. Isolation and Characterization of Oval Cells and Comparison with Other Liver Cell Types from Normal and Carcinogen-Treated Rats III. Growth of Liver Epithelial Cells in Vitro IV. Perspectives References4. Isolation and Characterization of Lymphocytes from Mature Mouse Liver I. Introduction II. Preparation of Suspensions of Cells from Liver III. Separation of Lymphocytes from Mouse Liver IV. Further Characterization of Lymphocytes from Liver V. Concluding Remarks References5. A Comparison of Cell Separations Obtained with Centrifugal Elutriation and Sedimentation at Unit Gravity I. Introduction II. A Theoretical Comparison and Factors Influencing Separation III. Applications IV. Summary and Conclusions References6. Limiting Dilution Assays for the Separation, Characterization, and Quantitation of Biologically Active Particles and Their Clonal Progeny I. Introduction II. Sample LDAs: SHPM Validity Tests III. Sample LDAs: Sample Estimators IV. Population LDAs V. Assay Design VI. Comparative Experiments VII. Clonal Analysis VIII. Partition Analysis IX. Conclusion References7. Purification and Functional Evaluation of Human Polymorphonuclear Leukocytes I. Introduction II. Background III. Techniques for the Isolation of Polymorphonuclear Neutrophils IV. Considerations in Choosing a Technique for the Purification of Polymorphonuclear Neutrophils V. Functional Analysis of Circulating Human Polymorphonuclear Neutrophils Isolated by Different Methods VI. Concluding Remarks References8. Fabrication of a Manually Operable Countercurrent Distribution Apparatus and Its Application for Separation of Cells in Aqueous Two-Phase Polymer System I. Introduction II. Countercurrent Distribution (CCD) III. Separation of Rat Testicular Cells by Manually Operable CCD Apparatus IV. Conclusion References9. Separation and Characterization of Phagocytes from Human Colon I. Introduction II. Dissociation of Mucosal Cells III. Enrichment of Mucosal Phagocytes IV. Analysis of Cell Suspensions by Flow Cytometry V. Functional Analysis VI. Concluding Remarks References10. Exploitation of Surface Molecules for Separation of Cells from Mosaic Livers I. Introduction II. Construction of Genotypic Mosaic Livers III. Immunological Approaches Toward Purifying Donor- and Host-Origin Liver Cells from Genotypic Mosaic Livers IV. Summary References11. Isolation, Characterization, and Possible Functions of Follicular Dendritic Cells from Tonsils and Adenoids I. Introduction II. Characteristics of Follicular Dendritic Cells (FDC) in Situ III. Isolation of FDC IV. Characterization of Isolated FDC V. Functional Study of FCD in Vitro VI. Conclusions References12. Magnetite-Protein Conjugates for the Separation of Cells by High Gradient Magnetic Filtration I. Introduction II. Magnetic Filtration III. Particulate Magnetic Labels IV. Cell Labeling V. Cell Sorting VI. Summary References13. The Development of Techniques That Permit the Selection and Growth of Malignant Cells from Human Colonic Carcinomas I. Introduction II. Tissue Procurement and Initial Tissue Processing III. Human Colon Cancer Cell Lines IV.