Cell Separation

ContributorsPrefaceContents of Previous Volumes1. Separation of Malignant Lymphoid Cells by Countercurrent Distribution I. Introduction II. Experimental Methods III. Fractionation of RAW117 Lymphoma Cells IV. Discussion of the Technique References2. Use of Lectins for Separation of Cells I. Introduction II. Methodology III. Selected Applications IV. Concluding Remarks References3. Enzymatic Disaggregation of Human Bronchogenic Carcinomas followed by Velocity Sedimentation of Cells I. Introduction II. Enzymatic Disaggregation of Tumors III. Suspensions of Tumors: Storage and Composition IV. Purification of Cells from Lung Tumors V. Concluding Remarks References4. Macrophage Electrophoretic Migration (MEM) Test with Some Illustrative and Heuristic Applications I. Historical Introduction II. Basic Requirements III. Preparation of Macrophages IV. Preparation of Lymphocytes V. Procedure: Cancer VI. Applications References5. Implementation of Microtechniques to Resolve Tissue Heterogeneity I. Introduction II. Description of Microtechniques III. Cell Differentiation in the Cellular Slime Mold Dictyostelium discoideum IV. Adaptation of the Micromethods to Human Mammary Tumors V. Conclusions References6. In Vitro Isolation of Malignant Cells from Small Cell Carcinomas I. Introduction II. Establishment of Cell Lines III. Characteristics of Cultured Cells IV. Summary References7. Computer-Controlled Cell Electrophoresis Microscope I. Introduction II. Design of the Automated Electrophoresis Microscope System (AEMS) III. Features Important to the Operation of the AEMS IV. Conclusions References8. Separation of Cells from the Rat Anterior Pituitary Gland I. Cellular Organization of the Rat Anterior Pituitary Gland II. Preparation of Rat Pituitary Cell Suspensions III. Methods Used to Differentiate Pituitary Cell Types IV. Review of Pituitary Cell Separation Studies V. Separated Pituitary Cells: Applications VI. Concluding Remarks References9. Isolation of Type II Pneumocytes and Their Application in the Study of Pulmonary Metabolism I. Introduction II. Overview-Type II Cell Isolation III. Method of Isolation IV. Applications V. Conclusion References10. Selective Media I. Introduction II. Biological Basis for Selective Growth III. Families of Cells with Similar Growth Requirements IV. Selective Growth of Human Epidermal Keratinocytes V. Techniques for Achieving Selective Growth VI. Future Prospects References11. Cell Labeling and Separation Using Immunospecific Microspheres I. Introduction II. General Considerations III. Cell-Specific Ligands IV. Microspheres for Cell Labeling and Separation V. Cell-Labeling Methods VI. Applications of Microspheres in Cell Separations VII. Summary and Concluding Remarks References12. Collagenase and the Isolation of Cells from Bone I. Cells of the Osteoblast Line II. Cells of the Osteoclast Line III. Isolation of Calvarial Cells IV. Clostridium histolyticum Collagenase V. Cell Separation Techniques VI. Culture of Human Bone Cells VII. Freezing Cells for Storage References13. Cell Separation by Biological Methods I. Introduction II. Differential Culture Procedures III. Selective Reagents IV. Selective Media V. Discussion ReferencesIndex