Cell Separation

ContributorsContents of Volume 1Preface1. Separation of Parafollicular Cells from Thyroid Follicular Cells by Affinity Chromatography Using Thyroglobulin-Coupled Sepharose I. Thyroid Structure and Function II. Parafollicular Cells and Serotonin III. Obtaining Parafollicular Cells for Study IV. Serotonin Binding Protein V. Distribution of Parafollicular Cells VI. Dissociation of the Cells of the Thyroid VII. Separation of Parafollicular Cells References2. Procurement and Purification of Eosinophils I. Introduction II. Procurement of Eosinophils III. Purification IV. Summary and Conclusions References3. Experimental Factors Involved in Separation by Centrifugal Elutriation I. Introduction II. Methodology of Elutriation III. Concluding Remarks References4. Analysis and Separation of Stromal Cells Infiltrating Tumors I. Introduction II. Previous Work III. Quantitation and Subcompartments IV. Identification of Stromal Cells and Morphometry V. Purification of Cells from Tumors VI. Concluding Comments References5. Selective Isolation of Epithelial Cells in Primary Expiant Cultures of Human and Animal Tissues I. Introduction II. Initiation of Primary Expiant Cultures III. Selective Isolation of Epithelial Cells IV. Identification of Human Bronchial and Bladder Epithelial Cells in Primary Expiant Cultures V. Discussion VI. Other Techniques References6. Isolation of Human Natural Killer Cells (Large Granular Lymphocytes) I. Introduction II. Isolation of Large Granular Lymphocytes (LGL) III. Comments References7. Purification of Islets and Cells from Islets I. Introduction II. Isolation of Pancreatic Islets III. Islet Purification IV. Preparation of Islet Cells V. Separation of Islet Cell Subpopulations VI. Summary References8. Magnetic Cell Sorting I. Introduction II. Magnetism and Magnetic Forces III. High Gradient Magnetic Separation IV. Magnetic Particles for Cell Labeling V. Conclusions References9. Separation of Cells by Preparative Density Gradient Electrophoresis I. Introduction II. Electrophoresis Theory III. Preparative Cell Electrophoresis in Density Gradients IV. Separation and Characterization of Cells by Density Gradient Electrophoresis V. Concluding Remarks References10. Isolation, Characterization, and Culture of Human Mammary Epithelial Cells I. Introduction II. Isolation of Mammary Epithelial Cells in Culture III. Method for the Culture of Human Mammary Epithelial Cells IV. Verification of the Mammary Epithelial Origin of the Cultured Cells V. Verification of the Malignant Origin of the Epithelial Cells Derived from Tumor Specimens VI. A Marker of Early Stages in Malignant Progression VII. Use of the Clonal Assay to Study Chemotherapeutic Drug Sensitivity VIII. Summary References11. Solid-Phase Fractionation of Lymphoid Cells on Ligand-Coated Plastic Plates I. Introduction II. Lymphocyte Fractionation III. Isolation of Surface Immunoglobulin-Positive Lymphocytes IV. Isolation of Human Fc Receptor-Bearing Lymphocytes on Antigen-Antibody Coated Dishes V. Isolation of Fc Receptor-Bearing Human T Lymphocytes VI. Isolation of Antigen-Specific Lymphocytes VII. Isolation of Lymphocyte Subsets with Monoclonal Antibodies VIII. Summary References12. Large Capacity Separation of Cells in a Reorienting Zonal Rotor I. Introduction II. General Characteristics of Reorienting Zonal Rotors III. Types of Separations Carried out in the TX-28 Rotor IV. Choice of Reorienting Zonal Rotor Versus Other Techniques V. Concluding Comments References13. Short-Term Culture of Cells from Human Solid Tumors in Semisolid Medium I. Introduction II. Methodology for Clonogenic Assays III. Conclusions References14.