Cell Separation

ContributorsPreface1. Methods for Obtaining Cells in Suspension from Animal Tissues I. Introduction II. Nonenzymatic Methods of Tissue Disaggregation III. Proteolytic Enzymes for Disaggregation IV. Subsidiary Enzymes V. Protease Inhibitors VI. Protective Agents VII. Concluding Remarks References2. Evaluation of Data, Problems, and General Approach I. Introduction II. Markers III. Quantitative Characterization of Cells Before and After Cell Separation IV. Morphological Criteria V. Concluding Comments References3. Sedimentation of Cells: An Overview and Discussion of Artifacts I. Introduction II. Theory III. Velocity Sedimentation IV. Isopycnic Sedimentation V. Comparison of Velocity and Isopycnic Sedimentation VI. Differential Sedimentation and Discontinuous Gradients VII. Artifacts in Sedimentation of Cells VIII. Concluding Remarks References4 . Separation of Host Cells Infiltrating Tumors and Allografts by Velocity Sedimentation at Unit Gravity I. Introduction II. Theoretical Considerations III. Potential Problems and Practical Limitations IV. Velocity Sedimentation of Dissociated Animal Tumors v. Velocity Sedimentation of Ascites Tumors, Leukemias, and Lymphomas VI. Velocity Sedimentation of Disaggregated Human Neoplasms VII. Recovery of Allograft-infiltrating Host Cells by 1 g Velocity Sedimentation VIII. Concluding Remarks References5. Analytical Characterization of Adult Granulocyte-Macrophage Progenitor Cells by Sedimentation Velocity and Buoyant Density I. Introduction II. Purification Studies III. Characterization of the Clonable Mouse Granulocyte-Macrophage Progenitor Cells (CFU-C) IV. Relationship of Mouse CFU-C to Other Clonable Hemopoietic Precursors and Stern Cells V. Physical Properties of Human CIonable Granulocyte and Macrophage Progenitor Cells VI. Conclusions References6. Sedimentation of Cells in Colloidal Silica (Percoll) I. Introduction II. Properties of Percoll III. Principles of Cell Separation in Percoll IV. Practical Aspects of the Use of Percoll v. Does Percoll Interfere with Cell Functions? VI. Future Use of Percoll References7. Separation of Different Kinds of Nucleated Cells from Blood by Centrifugal Elutriation I. Introduction II. Physical Characteristics of Human Blood Leukocytes III. Practical Technique IV. Isolation of Lymphocytes V. Isolation of Monocytes VI. Isolation of Granulocytes VII. Concluding Remarks References8. A New Approach to the Separation of Cells at Unit Gravity I. Introduction II. Theory III. CelSep Apparatus for Unit Gravity Separations IV. Standard Separation Procedure V. Separation of Human Monocytes from Blood VI. Characterization of Human Myeloid Stem Cells VII. Fractionation of Canine Gastric Cells VIII. Advantages and Disadvantages of the CelSep Method References9. Electronic Cell Sorting of Hemopoietic Progenitor Cells I. Introduction II. General Considerations III. Sorting of Unstained Hemopoietic Cells IV. Sorting of Hemopoietic Cells Labeled with Antibodies V. Sorting of Hemopoietic Cells Labeled with Lectins VI. Sorting of Hemopoietic Cells by Other Techniques VII. Summary and Future Perspectives References10. Separation of Individual Cells from the Fundic Gastric Mucosa I. Introduction II. Methods for Gastric Mucosal Cell Isolation III. Methods for Cell Purification IV. General Characteristics of Isolated Gastric Cells V. Specific Advantages and Disadvantages of the Various Purification Methods VI. Functional Characterization of Isolated Gastric Cells References11. Isolation and Culture of Homogeneous Populations of Glomerular Cell Types I. Introduction II. Background III. Techniques for Isolating Glomerular Cells IV.