Methods in Cell Physiology

List of ContributorsPreface1. Survey of Cytochemistry I. Survey II. Summary and Conclusions References2. Methods of Culture for Plasmodial Myxomycetes I. Introduction II. Culture of Impure Myxomycètes III. Isolation and Pure Culture IV. Axenic Culture of Physarum polycephalum on Soluble Media V. Sporulation (Fruiting) of Physarum polycephalum in Pure Culture VI. Sclerotia and Spherule Formation VII. Changes Observed after Prolonged Growth in Pure Culture References3. Mitotic Synchrony in the Plasmodia of Physarum Polycephalum and Mitotic Synchronization by Coalescence of Microplasmodia I. Introduction II. Life History of Physarum Polycephalum III. The Experimental Material IV. Discussion V. Summary References4. Introduction of Synchronous Encystment (Differentiation) in Acanthamoeba sp. I. Introduction II. The Encystment Process: A Description III. Induction of Synchronous Encystment: The Method IV. Optimal Conditions for Synchronous Encystment V. Evaluation of the Method References5. Experimental Procedures and Cultural Methods for Euplotes Eurystomus and Amoeba Proteus I. Introduction II. Euplotes Eurystomus III. Amoeba Proteus References6. Nuclear Transplantation in Ameba I. Introduction II. Equipment III. Operating Procedure IV. Conclusion References7. Experimental Techniques with Ciliates I. Introduction II. Experimental Possibilities with Stentor III. Techniques with Paramecium References8. Methods for Using Tetrahymena in Studies of the Normal Cell Cycle I. Introduction II. Special Equipment III. Manipulation of Cells References9. Continuous Synchronous Cultures of Protozoa I. Introduction II. Light-Induced Synchrony in Euglena Gracilis III. Temperature-Induced Synchrony in Astasia Longa IV. Concluding Remarks References10. Handling and Culturing of Chlorella I. Introduction II. The Organisms and Their Growth III. The Culture of Chlorella Appendix References11. Culturing and Experimental Manipulation of Acetabularia I. Introduction to the Organism II. Culture Techniques III. Microsurgery IV. Biochemical Analysis V. Potentialities and Limitations References12. Handling of Root Tips I. Introduction II. Germination of Seeds and Growing of Roots III. Fixation of Root Tips IV. Staining of Cells V. Making Slides Permanent VI. Autoradiographic Methods VII. Isolation of Nuclei VIII. Special Uses IX.Conclusion References13. Grasshopper Neuroblast Techniques I. Introduction II. Selection of Species III. Collecting IV. Rearing V. Eggs VI. Development VII. Culture Techniques VIII. Separated Cell Techniques IX. Fixed Cell Techniques X. Radiation Techniques XI. Chemical Techniques XII. Gas Techniques XIII. Microdissection References14. Measurement of Material Uptake by Cells: Pinocytosis I. Introduction II. Demonstration of Pinocytosis III. Inducers of Pinocytosis IV. Quantitative Estimation of Pinocytic Uptake V. Significance of the Values Obtained and Errors Likely to Be Encountered in the Different Methods References15. Quantitative Autoradiography I. Introduction II. Theoretical Considerations III. Practical Considerations and Techniques IV. The Problem of "Intermediates" References16. High-Resolution Autoradiography I. Introduction II. Methods III. Discussion of the Methods IV. Resolution V. Discussion References17. Autoradiography with Liquid Emulsion I. Introduction II. Emulsions III. Procedure References18. Autoradiography of Water-Soluble Materials I. Introduction II. Procedures III. Experimental Example References19. Preparation of Mammalian Metaphase Chromosomes for Autoradiography I.