Methods in Cell Physiology
Volume 3
Published on 13. June 2016
404 pages
978-1-4832-2484-8 (ISBN)
Description
Methods in Cell Physiology
More details
Content
- Front Cover
- Methods in Cell Physiology
- Copyright Page
- Table of Contents
- List of Contributors
- Preface
- Contents of Previous Volumes
- Chapter 1. Measurement of Cell Volumes by Electric Sensing Zone Instruments
- I. Introduction
- II. Design of Equipment
- III. Evaluation of Fidelity of Measurement of Particle Volume
- IV. Causes and Remedies of Distortion in Electric Sensing Zone Volume Measurements
- Acknowledgments
- References
- Chapter 2. Syncbronization Methods for Mammalian Cell Cultures
- I. Introduction
- II. Cell Reproductive Cycle
- III. Synchrony by Analysis
- IV. Synchrony by Inhibition and Release of DNA Synthesis
- V. Synchrony by Selection of Mitotic Cells
- VI. Factors Affecting the Loss of Synchrony
- VII. Multiple Synchronization
- References
- Chapter 3. Experimental Techniques for Investigation of the Amphibian Lens Epithelium
- I. Introduction
- II. Methods for Fixed Material
- III. Methods for Live Material
- IV. Assessment of Isozyme Activity
- V. Concluding Remarks
- Acknowledgment
- References
- Chapter 4. Cultivation Tissues and Leukocytes from Amphibians
- I. Introduction
- II. Culture Technique for Adult Tissues
- III. General Aspects of Cultured Cells in Vitro
- IV. Leukocyte Culture of Amphibians
- V. Conclusions
- Acknowledgments
- References
- Chapter 5. Experimental Procedures for Measuring Cell Population Kinetic Parameters in Plant Root Meristems
- I. Introduction
- II. Definition of Terms
- III. Model of a Hypothetical Root Meristem
- IV. Determination of the Mitotic Cycle Duration
- V. Determination of the Mitotic Cycle and Its Periods-The Use of Tritiated Thymidine
- VI. The Combined Use of Colchicine and Tritiated Thymidine as Cell Markers
- VII. Determination of the Number of Proliferating Cells
- VIII. Detailed Methodology
- Acknowledgments
- References
- Chapter 6. Induction of Synchrony in Chlamydomonas moewusii as a Tool for the Study of Cell Division
- I. Introduction
- II. Description of the Organism
- III. Method for Maintaining and Culturing Cells
- IV. Induction of Synchrony
- References
- Chapter 7. Staging of the Cell Cycle with Time-Lapse Photography
- I. Introduction
- II. General Methods
- III. Staging and Analysis of the Cell Cycle
- References
- Chapter 8. Method for Reversible Inhibition of Cell Division in Tetrahymena pyriformis Using Vinblastine Sulfate
- I. Introduction
- II. Growth of Cells
- III. Inhibition of Cell Division
- IV. Recovery from Inhibition
- V. Concluding Remarks
- References
- Chapter 9. Physiological Studies of Cells of Root Meristems
- I. Introduction
- II. Growing Roots
- III. Culture of Roots and Root Cells
- IV. Use of Isotopically Labeled Precursors
- V. Treatments with Drugs and Antimetabolites
- VI. Growth Factors-Auxins and Cytokinins
- VII. Radiation Effects
- VIII. Fixation and Staining
- IX. Conclusions
- Acknowledgments
- References
- Chapter 10. Cell Cycle Analysis
- I. Introduction
- II. Experimental Methods for Obtaining Cell Cycle Data
- III. Analysis of Empirical Data-Conversion of Fractions of Cells in a Phase to the Duration of the Phase
- IV. Review of Generation-Time and Phase-Duration Distributions
- V. Effect of the Distribution of Generation Times on the Empirical Determination of Generation Times
- VI. Effect of G2-Duration Distributions and Mitotic Durations on Percentage-Labeled Mitoses Curves
- VII. Methods for Determining the Durations of Mitotic Phases in Vivo
- VIII. Statistical Considerations in the Determination of Generation- Time and Phase-Duration Distributions
- IX. Summary
- References
- Chapter 11. A Method for the Study of Cell Proliferation and Renewal in the Tissues of Mammals
- I. Introduction
- II. Assumptions in the Use of Tritiated Thymidine for Studies of Cell Renewal
- III. Choice of a Route of Tritiated Thymidine Administration
- IV. Description of the Method
- V. Results and Discussion of the Method
- VI. Possible Application of the Method for Future Studies
- VII. Summary
- Acknowledgments
- References
- Chapter 12. Isohtion and Fractionation of Metaphase Chromosomes
- I. Introduction
- II. Accumulation of Cells in Metaphase Arrest
- III. Hypotonie Swelling
- IV. Rupture of Hypotonically Swollen HeLa Cells
- V. Elimination of Contaminants from Ruptured HeLa Cells
- VI. Properties of Purified HeLa Chromosomes
- VII. Isolation and Fractionation of Chinese Hamster (CH) Chromosomes
- VIII. Hypotonie Treatment and Cell Rupture
- IX. Fractionation of Chromosomes and Elimination of Other Cellular Constituents by Sucrose Gradient Sedimentation
- X. Discussion
- References
- Chapter 13. Autoradiography with the Electron Microscope: Properties of Photographic Emulsions
- I. Introduction
- II. Techniques for Autoradiography with the Electron Microscope
- III. Theoretical Considerations
- IV. Determination of the Sensitivity of Photographic Emulsions
- V. Discussion
- Acknowledgments
- References
- Chapter 14. Cytological and Cytochemical Methodology of Histones
- I. Introduction
- II. Cytochemical Methods
- III. Concluding Remarks
- Acknowledgments
- References
- Chapter 15. Mitotic Cells as a Source of Synchronized Cultures
- I. Introduction
- II. Equipment and Procedures
- III. Selection of Mitotic Populations
- IV. Summary
- References
- AUTHOR INDEX
- SUBJECT INDEX
