Methods in Cell Physiology
Volume 2
Published on 15. June 2016
444 pages
978-1-4832-2483-1 (ISBN)
Description
Methods in Cell Physiology
More details
Content
- Front Cover
- Methods in Cell Physiology
- Copyright Page
- Table of Contents
- List of Contributors
- Preface
- Contents of Previous Volume
- Chapter 1. Nuclear Transplantation in Amphibia
- I. Introduction
- II. Nuclear Transplantation in Anurans
- III. Nuclear Transplantation in Urodeles
- IV. Differences in the Methods of Amphibian Nuclear Transplantation
- V. Some Applications of the Methods of Amphibian Nuclear Transplantation
- VI. Concluding Remarks
- ACKNOWLEDGMENTS
- REFERENCES
- Chapter 2. Techniques for the Study of Lampbrush Chromosomes
- I. Introduction
- II. Animals and Their Care
- III. Routine Preparations for Visual Microscopy
- IV. Additional Techniques
- V. Materials
- ACKNOWLEDGMENT
- REFERENCES
- Chapter 3. Micrurgy on Cells with Polytene Chromosomes
- I. Introduction
- II. General Procedures and Instrumentation for Micrurgy
- III. Cell Types with Polytene Chromosomes
- Their Handling and Culture
- IV. Isolation of Nuclei and Chromosomes
- V. Removal of Chromosome Segments and Whole Chromosomes
- VI. Transplantation of Cytoplasm and Chromosomes
- VII. Microinjection
- VIII. Conclusions
- Appendix
- ACKNOWLEDGMENTS
- REFERENCES
- Chapter 4. A Novel Method for Cutting Giant Cells to Study Viral Synthesis in Anucleate Cytoplasm
- I. Introduction
- II. X-Ray-Induced Giant HeLa Cells
- III. The Glass-Cutting Wheel Technique for Micrurgy
- IV. Viral Synthesis in Anucleate Cytoplasm
- V. Conclusions
- ACKNOWLEDGMENTS
- REFERENCES
- Chapter 5. A Method for the Isolation of Mammalian Metaphase Chromosomes
- I. Introduction
- II. Preparation of Cells
- III. Chromosome Extraction
- IV. Some Properties of the Isolated Chromosomes
- V. Discussion
- ACKNOWLEDGMENTS
- REFERENCES
- Chapter 6. Isolation of Single Nuclei and Mass Preparations of Nuclei from Several Cell Types
- I. Introduction
- II. Isolation of Individual Macronuclei of Euplotes eurystomus
- III. Isolation of Individual Nuclei of Amoeba proteus
- IV. Isolation of Macronucleus of Stentor coeruleus
- V. Isolation of Macronuclear Anlagen of Euplotes woodruffi
- VI. Isolation of Micro- and Macronuclei of Paramecium caudatum
- VII. Isolation of Macronuclei of Other Ciliates
- VIII. Mass Isolation of Tetrahymena Nuclei
- IX. Nuclear Isolation in S3 HeLa Cells
- REFERENCES
- Chapter 7. Evaluation of Turgidity, Plasmolysis, and Deplasmolysis of Plant Cells
- I. Introduction
- II. Methods for Measurement of the Osmotic Ground Value (Og)
- III. Determination of the Suction Potential of the Cell Contents in Incipient Plasmolysis (Sig)
- IV. Methods for Measurement of the Suction Potential of the Cell Contents in the Normal State (Sin)
- V. Measurement of the Suction Potential of the Cell in the Normal State (Szn)
- VI. Determination of the Passive Permeability of the Plant Cell with the Plasmolytic Method
- VII. Evaluation of Wall Attachment (Adhesion) and Protoplasmic Viscosity
- VIII. Cytomorphological Alterations Generated by Plasmolysis
- IX. Plasmolysis and Turgidity as Test Method for the Living State of the Plant Cell
- REFERENCES
- Chapter 8. Culture Media for Euglena gracilis
- I. Introduction
- II. Maintenance
- III. Autotrophic Media
- IV. Heterotrophic Acidic Medium
- V. Neutral and Alkaline Media
- VI. General Comments
- REFERENCES
- Chapter 9. General Area of Autoradiography at the Electron Microscope Level
- I. Introduction
- II. Factors in Autoradiographic Technique
- III. Specimen Preparation
- IV. Concluding Remarks
- ACKNOWLEDGMENT
- REFERENCES
- Chapter 10. High Resolution Autoradiography
- I. Introduction
- II. Light Microscope Techniques
- III. Electron Microscope Techniques
- REFERENCES
- Chapter 11. Methods for Handling Small Numbers of Cells for Electron Microscopy
- I. Introduction
- II. Agar Embedding
- III. Flat Embedding
- IV. Embedding in a Capillary Tube
- V. Use of a Microcentrifuge
- VI. Conclusion
- REFERENCES
- Chapter 12. Analysis of Renewing Epithelial Cell Populations
- I. Introduction
- II. Preparation and Handling of Tissues for Autoradiography
- III. Assumptions in the Use of Labeled Thymidine
- IV. Determination of the Progenitor Cell Cycle by Double Labeling with Thymidine-H3 and Thymidine-C14
- V. Analysis of Renewing Epithelia Using Single Injection of Thymidine-H3
- VI. Measurement of Tissue Turnover and Transit Times
- VII. Relationship between Cell Proliferation and Specialization in Adult Renewing Epithelia
- ADDENDUM
- ACKNOWLEDGMENTS
- REFERENCES
- Chapter 13. Patterns of Cell Division: The Demonstration of Discrete Cell Populations
- I. Introduction
- II. Demonstration of G1 and G2 Cell Populations in Mouse Epidermis
- III. Demonstration of G1 and G2 Cell Populations in Other Experimental Systems
- IV. Additional Procedures for Detecting G2 Cell Populations
- V. Methods for Detecting "Physiological Subpopulations"in Mouse Ear Epidermis in Vitro
- VI. General Conclusions and Over-all Concept of Discrete Cell Populations
- REFERENCES
- Chapter 14. Biochemical and Genetic Methods in the Study of Cellular Slime Mold Development
- I. Introduction
- II. Maintenance and Preservation of Stocks
- III. Preparation of Cells for Developmental Studies
- IV. Isolation of Nucleic Acids
- V. Analyses of Cell Constituents
- REFERENCES
- Author Index
- Subject Index
