Animal Cell Biotechnology
Methods and Protocols
4th Edition
Published on 19. December 2019
XIV, 399 pages
978-1-0716-0191-4 (ISBN)
Description
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition
constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
More details
Series
Edition
Fourth Edition 2020
Language
English
Place of publication
New York
United States
Publishing group
Springer US
Target group
Professional and scholarly
Product notice
Reflowable
Illustrations
XIV, 399 p. 127 illus., 80 illus. in color.
ISBN-13
978-1-0716-0191-4 (9781071601914)
DOI
10.1007/978-1-0716-0191-4
Schweitzer Classification
Other editions
Additional editions
Book
12/2019
4th Edition
Springer
€181.89
Shipment within 15-20 days
Content
Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation.- Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays.- Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment.- ambr® 15 Microbioreactors for CHO Cell Cultivation.- Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications.- HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR).- Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode.- Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors.- Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production.- How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.- Generic Workflow for the Setup of Mechanistic Process Models.- Estimation of Process Model Parameters.- Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments.- Design, Optimization, and Adaptive Control of Cell Culture Seed Trains.- High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays.- Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture.- Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells.- Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples.- An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures.- Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures.- Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method.- Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System.
