Phosphatases
Academic Press
Published on 24. August 2018
440 pages
978-0-12-813882-3 (ISBN)
Description
Methods in Enzymology, Volume 607: Phosphatases, the latest release in this ongoing series, highlights new advances in the field as detailed by an international board of authors. This latest release includes chapters on Empirical Valence Bond Simulations of the Evolution of Enzyme Function, QM/MM Free Energy and Kinetic Isotope Analysis of Phosphoryl Transfer in Enzymes, the Structural, Mechanism and Evolution of Phosphatases, How to Define Rapid Motions in Pumping Pyrophosphatases, The Evolution of K+-Independence in Pyrophosphatases, the Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases, and an Investigation of Nucleotide Loading and Effector Binding of K-Ras.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
- Updated release includes the latest information on phosphatases
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Content
1. Empirical Valence Bond Simulations of Organophosphate Hydrolysis: Theory and PracticeMiha Purg and Shina Caroline Lynn Kamerlin2. Analysis of Phosphoryl-Transfer Enzymes With QM/MM Free Energy SimulationsDaniel Roston, Xiya Lu, Dong Fang, Darren Demapan and Qiang Cui3. Defining Dynamics of Membrane-Bound Pyrophosphatases by Experimental and Computational Single-Molecule FRETSteven P.D. Harborne, Jannik Strauss, Ainoleena Turku, Matthew A. Watson, Roman Tuma, Sarah A. Harris and Adrian Goldman4. A Simple Strategy to Determine the Dependence of Membrane-Bound Pyrophosphatases on K+ as a CofactorJannik Strauss, Craig Wilkinson, Keni Vidilaseris, Steven P.D. Harborne and Adrian Goldman5. Crystallization of Liganded Phosphatases in the HAD Superfamily Christine Harvey, Katherine O'Toole and Karen N. Allen6. Structure, Mechanism, and Substrate Profiles of the Trinuclear Metallophosphatases From the Amidohydrolase SuperfamilySwapnil V. Ghodge and Frank M. Raushel7. Affinities and Comparisons of Enzyme States by Principal Component Analysis of NMR Spectra, Automated Using TREND SoftwareJia Xu and Steven R. Van Doren8. Assessment and Impacts of Phosphorylation on Protein Flexibility of the a-D-PhosphohexomutasesKyle M. Stiers and Lesa J. Beamer9. Chemical Tools for Studying the Impact of cis/trans Prolyl Isomerization on Signaling: A Case Study on RNA Polymerase II Phosphatase Activity and SpecificityNathaniel Tate Burkholder, Brenda Medellin, Seema Irani, Wendy Matthews, Scott A. Showalter and Yan Jessie Zhang10. Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine KinasesRong Gao and Ann M. Stock11. Measuring the Activities of Two-Component Regulatory System PhosphatasesRobert B. Bourret and Ruth E. Silversmith12. Assembly of PGAM5 Into Multimeric Complexes Provides a Mechanism for Allosteric Regulation of Phosphatase ActivityPeter Tipton, Tong Su and Mark Hannink13. Purification of Lipin and Measurement of Phosphatidic Acid Phosphatase Activity From LiposomesMitchell E. Granade and Thurl E. Harris14. Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase ActivitiesLindsey C. Szymczak, Che-Fan Huang, Eric J. Berns and Milan Mrksich15. Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase RegulationDaniel R. Dempsey and Philip A. Cole
