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This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical,
Vaccine Delivery Technology: Methods and Protocols
is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful fieldof vaccines.
Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Series
Language
Place of publication
Publishing group
Target group
Professional and scholarly
Product notice
Illustrations
XV, 590 p. 149 illus., 127 illus. in color.
File size
ISBN-13
978-1-0716-0795-4 (9781071607954)
DOI
10.1007/978-1-0716-0795-4
Schweitzer Classification
Vaccine Delivery and Immune Response Basics.- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes.- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed.- Antigen Discovery in Bacterial Pan-Proteomes.- Alphavirus-Based Antigen Preparation.- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System.- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens.- Production of Zika Virus Virus-Like Particles.- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs).- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines.- Whole-Cell Vaccine Preparation: Options and Perspectives.- O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein.- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines.- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines.- Attenuation Methods for Live Vaccines.- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism.- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.- Lipofection-Based Delivery of DNA Vaccines.- Flavivirus DNA Vaccine Design and Adjuvant Selection.- Vaccine Delivery with a Detoxified Bacterial Toxin.- Needle-Less or Non-Invasive Delivery Technology.- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.- A Hybrid Biological-Biomaterial Vector for Antigen Delivery.- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens.- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder.- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice.- The Quantification of Antigen-Specific T Cells by IFN-? ELISpot.- The Measurement of Antigen-Specific IgG Titers by Direct ELISA.- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model.- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils.- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.
