Recent Advances in Steroid Biochemistry

Front CoverRecent Advances in Steroid BiochemistryCopyright PageChapter 1. Partial Purification of Steroid-Receptor Complexes by DNA-Cellulose Chromatography and Isoelectric Focusing Introduction Experimental Results and Discussion ReferencesChapter 2. Enzymatic Techniques in Steroid Assay Introduction Experimental Method Results Discussion ReferencesChapter 3. Mass Fragmentography of Steroid Hormones Introduction Experimental Results and Discussion Conclusions References DiscussionChapter 4. Separation and Computerized Gas Chromatography-Mass Spectrometry of Unconjugated Neutral Steroids in Plasma Introduction Experimental Results Discussion ReferencesChapter 5. Steroids as Haptens: Optimal Design of Antigens for the Formation of Antibodies to Steroid Hormones Definition of the Problem and Rationale of Approach General Methods Properties of Antisera to Gonadal Steroids in Relation to Structure of the Antigenic Conjugate Concluding Remarks ReferencesChapter 6. Some Aspects of the Use of 125i-Labelled Ligands for Steroid Radioimmunoassay Introduction Experimental Results Discussion ReferencesChapter 7. Testosterone Binding Capacity in Relation to the Production and Metabolism of Testosterone in Dogs, Experiences of A New Method Introduction Materials and Methods Results Discussion ReferencesChapter 8. Simultaneous Estimation of Testosterone, Progesterone and Androstenedione by Gas Chromatography-Mass Spectrometry with A Single Ion Detection Correlation with Radioimmunoassay Introduction Discussion ReferencesGeneral DiscussionChapter 9. The Preparation and use of Purified and Unpurified Dispersed Adrenal Cells and a Study of the Relationship of Their Camp and Steroid Output Introduction Experimental and Results Discussion Appendix ReferencesChapter 10. Biological Consequences of 18-Hydroxylation Introduction Experimental Results Discussion ReferencesChapter 11. Multifactorial Regulation of the Final Steps of Aldosterone Biosynthesis in the Rat Stimulators of An Early Biosynthetic Step Variable Steroidogenic Response to Stimulators Alterations in the Activity of Enzymes Involved in the Final Steps of Aldosterone Biosynthesis Renin-Angiotensin Potassium Serotonin Pituitary Gland and ACTH Conclusions References DiscussionChapter 12. Some Comparative Studies in Adrenocortical Steroidogenesis: An Interpretation of the Functional Homologies of the Mammalian and Non-Mammalian Adrenal Cortex Introduction Materials and Methods Results Discussion ReferencesChapter 13. Steroidogenesis in Adrenal Cells Introduction Results and Discussion References DiscussionChapter 14. Stimulation Factors for Cholesterol Side-Chain Cleavage in the Corpus Luteum and Adrenal Gland Introduction Methods Results Discussion ReferencesChapter 15. In Vitro Study On Corticosterone Cytochrome P-450 Binding in Relation to the Regulation of Corticosterone Metabolism Introduction Materials and Methods Results Discussion ReferencesGeneral DiscussionChapter 16. Superfusion of Rat Testes With Mixtures of Labeled Testosterone and Androstenedione Introduction Experimental Results Discussion ReferencesChapter 17. Regulation of Steroidogenesis in Testis Introduction Experimental Results Discussion ReferencesChapter 18. Androgen Binding Proteins in Different Testis Compartments Preparation of Samples Sertoli Cell Production of ABP Regulation of ABP Production Androgens and Spermatogenesis ReferencesChapter 19. Sexual Differentiation of Hepatic Steroid Metabolism in the Rat Introduction Experimental Results and Discussion References DiscussionChapter 20.