Flow Cytometry and Cell Sorting
Published on 30. January 2019
247 pages
978-1-77361-770-1 (ISBN)
Description
Flow cytometry allows a cell population to be analysed. Thisbook describes the technology involved. This book covers managingflow cytometry/cell sorting core facilities for the emergingresearchers, in particular in developmental, cellular, and molecularbiology. This is a most useful book which will be a welcomeaddition for personal use and also for laboratories in a varietyof disciplines.
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Content
- Cover
- Half Title Page
- Title Page
- Copyright Page
- Declaration
- About the Editors
- Table of Contents
- List of Contributors
- List of Abbreviations
- Preface
- Chapter 1 Principles of Flow Cytometry
- 1.1 Introduction
- 1.2 Components of a Flow Cytometer
- 1.3 How Does Fluorescence Work?
- 1.4 Principles Underlying Portions of Flow Cytometry
- 1.5 Processing of Data
- 1.6 Gating
- 1.7 Sorting of Cells
- 1.8 Quality Control
- 1.9 A New Perspective
- 1.10 Conclusion
- 1.11 References
- Chapter 2 Flow Cytometry in the Realm of Bacteria
- 2.1 Introduction
- 2.2 Apparatus
- 2.3 Application of FC for Bacterial Analysis
- 2.4 Case Study: Antimicrobial Treatments and Flow Cytometry
- 2.5 Summary
- 2.6 References
- Chapter 3 Flow Cytometry: Applications
- 3.1 Introduction
- 3.2 Flow Cytometry: Principles
- 3.3 Clinical Applications of Flow Cytometry
- 3.4 Determining The Effectiveness of a Few Therapies
- 3.5 Flow Cytometry: Scope of Its Applications
- 3.6 Applications in Microbiology
- 3.7 Summary
- 3.8 References
- Chapter 4 Flow Cytometry in a Clinical Scenario
- 4.1 Introduction
- 4.2 Flow Cytometry in Microbiology
- 4.3 Milestones For Flow Cytometry in a Clinical Setting
- 4.4 Case Study: Flow Cytometry and Diagnosis
- 4.5 Overview of Applications in a Clinic
- 4.6 Flow Cytometry and Hematology
- 4.7 DNA Content Analysis by Flow Cytometry
- 4.8 Fluorochromes Used for Diagnosis
- 4.9 Quality Control for Clinical Diagnosis or Studies
- 4.10 Summary
- 4.11 References
- Chapter 5 Functional Virtual Flow Cytometry: A Visual Analytic Approach for Characterizing Single-Cell Gene Expression Patterns
- Abstract
- Background
- Methods
- Results
- Discussion and Conclusion
- Acknowledgments
- References
- Chapter 6 Detection of Low Abundance RNA Molecules in Individual Cells By Flow Cytometry
- Abstract
- Introduction
- Methods
- Results
- Discussion
- Acknowledgments
- Author Contributions
- References
- Chapter 7 Flow Cytometry Based Detection and Isolation of Plasmodium Falciparum Liver Stages in Vitro
- Abstract
- Introduction
- Materials and Methods
- Results
- Discussion
- Acknowledgments
- Author Contributions
- References
- Chapter 8 Applications of Flow Cytometry to Characterize Bacterial Physiological Responses
- Abstract
- Introduction
- Application of Flow Cytometry
- Concluding Remarks
- Acknowledgments
- References
- Chapter 9 Impedance Flow Cytometry: A Novel Technique in Pollen Analysis
- Abstract
- Introduction
- Material and Methods
- Results
- Discussion
- References
- Chapter 10 Flow Cytometry Bioinformatics
- Abstract
- Flow Cytometry Data
- Steps in Computational Flow Cytometry Data Analysis
- Data Preprocessing
- Identifying Cell Populations
- Diagnosis and Discovery
- Data Formats and Interchange
- Public Data and Software
- Open Problems
- Acknowledgments
- References
- Index
