Laser Capture Microdissection
Description
This volume details protocols that highlight the versatility of LCM across different biological contexts and analytical workflows. Chapters cover protocols on isolating cells from blood smears, live cell samples, and FFPE or frozen tissues. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Laser Capture Microdissection: Methods and Protocols aims to support both new and experienced researchers in effectively applying LCM to their own biological questions, across a wide variety of systems and technical platforms.
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Content
Laser capture microdissection from blood smears.- Live cell isolation by laser capture microdissection.- From Laser Microdissection to Spatial Glycomics: Lectin Microarray Protocols for Tissue Glycome Mapping with High-End Scanners.- Laser capture microdissection followed by DNA methylation profiling.- High resolution laser microdissection to investigate transcriptional states in formalin-fixed paraffin embedded samples.- Transcriptomic analysis from cryosectioned tissue samples using laser capture microdissection based Geo-seq.- Immunofluorescence imaging-guided laser microdissection for ultra-low input spatial tissue proteomics.- Low-Input Proteomics Protocols for Laser-Captured FFPE Samples with Orbitrap Astral DIA-MS.- Laser capture microdissection followed by histone H1 variant analysis by mass spectrometry.- A spatial proteomics approach to isolate and analyze amyloid proteins using laser capture microdissection and mass spectrometry.- Chromatin Immunoprecipitation-Sequencing and RNA-Sequencing from Laser Microdissected Human Lung Cancer Cells.- Laser capture microdissection followed by ATAC-seq.- Laser Capture Microdissection for quantitative shotgun lipidomics.- Artificial Intelligence-driven laser capture microdissection.- Automated Intelligent Microscopy for phenotypic identification, spatial localization and retargeting of cells.
