Synaptosomes
Published on 21. September 2018
XV, 312 pages
978-1-4939-8739-9 (ISBN)
Description
This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In
Neuromethods
series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.
Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.
More details
Series
Language
English
Place of publication
New York
United States
Publishing group
Springer US
Target group
Professional and scholarly
Product notice
Reflowable
Illustrations
XV, 312 p. 55 illus., 32 illus. in color.
File size
8,57 MB
ISBN-13
978-1-4939-8739-9 (9781493987399)
DOI
10.1007/978-1-4939-8739-9
Schweitzer Classification
Other editions
Additional editions
Content
Introduction to Synaptosome Methods and Applications.- Victor P. Whittaker: The Discovery of the Synaptosome and Its Implications.- Synaptosome Preparations: Which Procedure Should I Use?.- Protocol for a High-Throughput Semi-Automated Preparation for Filtered Synaptoneurosomes.- Subcellular Fractionation of Brain Tissue from Small Tissue Explants.- Preparation of P2 of Percoll-Purified Synaptosomes from Mammalian Brain Tissue.- Synaptosomal Bioenergetics and Glutamate Release.- Synaptosomes Bioenergetics and Calcium Handling: Aging Response.- Isolation of Highly Purified and Functionally Active Synaptic Mitochondria.- Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes.- Manipulating Synapse-Enriched Biochemical Fractions to Detect a Purified Synaptic Protein's Effect on Neurotransmitter Release.- Prosomes: Supramolecular Structures at the Synaptosome Membrane Involved in Vesicle Docking, Fusion,and Neurotransmitter Release.- SMPL-Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Cell Biology of Synapses.- Profiling of MicroRNA and Protein from Purified Synaptoneurosomes in a Neurodegenerative Disease Model.- Use of Synaptoneurosome Samples to Study Development and Plasticity of Human Cortex.- The Use of Synaptosomes in Studying Autism Spectrum Disorder and Other Neurodevelopmental Disorders.- Flow Cytometric Evaluation of Crude Synaptosome Preparation as a Way to Study Synaptic Alteration in Neurodegenerative Diseases.
