Fundamentals of Light Microscopy and Electronic Imaging
2nd Edition
Published on 22. August 2012
552 pages
978-1-118-38293-6 (ISBN)
Description
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.
The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.
PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website:
www.wiley.com/go/murphy/lightmicroscopy
Reviews / Votes
"This should be provided to all beginning graduatestudents entering microscopy labs. It describes the complicatedhardware of the system, while also explaining the physicsprinciples of microscopy on a simplistic level for basicbiologists. The authors achieve a perfect balance of theory andmethods." (Doody's, 15 November 2013) "It should be particularly useful to researchers gettingstarted in the field of microscopy as well as seasonedprofessionals. Summing Up: Highly recommended. Graduate students,researchers/faculty, and professionals/practitioners." (Choice, 1 October 2013) "In summary, Fundamentals of Light Microscopy, SecondEdition is a recommended starting point for the novice inmicroscopy and electronic imaging." (Journal ofBiomedical Optics, 1 February 2013)More details
Other editions
Additional editions
Douglas B. Murphy | Michael W. Davidson
Fundamentals of Light Microscopy and Electronic Imaging
Book
10/2012
2nd Edition
Wiley
€163.50
Shipment within 10-20 days
Software
09/2012
Wiley
€226.27
No shipping information available
Persons
DOUGLAS B. MURPHY supervises core facilities inmicroscopy and histology at the new HHMI Janelia Farm ResearchCampus in Ashburn, Virginia. An Adjunct Professor of Cell Biologyat Johns Hopkins School of Medicine in Baltimore, Maryland, Dr.Murphy helped establish the School of Medicine Microscope Facilitythere, which he supervised until 2006.
MICHAEL W. DAVIDSON is an assistant scholar/scientistaffiliated with the National High Magnetic Field Laboratory and theDepartment of Biological Science at Florida State University wherehe is involved in developing educational websites. His digitalimages and photomicrographs have graced the covers of over 2,000publications.
Content
Chapter 1: Fundamentals of Light Microscopy
Chapter 2: Light and Color
Chapter 3: Illuminators, Filters, and the Isolation of Specific Wavelengths
Chapter 4: Lenses and Geometrical Optics
Chapter 5: Diffraction and Interference in Image Formation
Chapter 6: Diffraction and Spatial Resolution
Chapter 7: Phase Contrast Microscopy and Darkfield Microscopy
Chapter 8: Properties of Polarized Light
Chapter 9: Polarization Microscopy
Chapter 10: Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy
Chapter 11: Fluorescence Microscopy
Chapter 12: Fluorescence Imaging of Dynamic Molecular Processes
Chapter 13: Confocal Laser Scanning Microscopy
Chapter 14: Two-Photon Excitation Fluorescence Microscopy
Chapter 15: Superresolution Imaging
Chapter 16: Imaging Living Cells With The Microscope
Chapter 17: Fundamentals of Digital Imaging
Chapter 18: Digital Imaging Processing
Appendix I
Appendix II
Appendix III
Glossary
References
Index
