The lac Operon
A Short History of a Genetic Paradigm
1st Edition
Published on 12. May 2011
IX, 207 pages
978-3-11-087947-6 (ISBN)
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09/1996
1st Edition
De Gruyter
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01/1996
1st Edition
De Gruyter
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Content
- Intro
- Introduction
- Part 1: A Short History of the lac System from its Beginning to 1978
- 1.1 From Noah to Pasteur: Adaptation in Yeast
- 1.2 Adaptation in Bacillus subtilis and Escherichia coli
- 1.3 Mutants in the lac System of Escherichia coli
- 1.4 Inducers of the lac System and the Discovery of Permease
- 1.5 Negative Control through Repressor
- 1.6 Further Evidence for Negative Control through Repressor
- 1.7 The Triumph of the Jacob-Monod Theory
- 1.8 Minor Defects in the Jacob-Monod Theory
- 1.9 Isolation of the Lac and ? CI Repressors
- 1.10 Putting the lac Genes on Phage f80 or ?
- 1.11 Lac and ? CI Repressors Bind to their Operator DNAs
- 1.12 Making Grams of Lac Repressor to Determine its Sequence
- 1.13 Mutations in lacI Suggest a Modular Structure of Repressor
- 1.14 Miller's Analysis of the I Gene: Climbing Mount Everest
- 1.15 Positive Control through the CAP Protein
- 1.16 Isolation and Sequence of lac Operator
- 1.17 Chemical DNA Sequencing
- 1.18 Steric Hindrance is the Mechanism of Repression
- 1.19 Chemical Synthesis of lac Operator DNA
- 1.20 Lac Repressor-Operator Complex Binds to Nitrocellulose
- 1.21 Polyacrylamide Gel Electrophoresis of Protein-DNA Complexes
- 1.22 Fusion of and Complementation within the lacZ Gene
- 1.23 Isolation of Lac Permease
- 1.24 The End of an Era
- Part 2: Misinterpretation
- 2.1 The Interpretation of Experiments is Sometimes Wrong
- 2.2 Adaptation Explained by Self-Replicating Genes
- 2.3 The Kinetics of Adaptation
- 2.4 Lac Repressor is RNA
- 2.5 Operator is RNA or Protein
- 2.6 Isolation of Lac Repressor
- 2.7 Isolation of Lac Permease and Arg Repressor
- 2.8 Genetic Proof that lac Operator is Dyadic
- 2.9 The Core of Lac Repressor Binds to lac Operator
- 2.10 Lac Repressor Uses ß-Sheet to Recognize lac Operator
- 2.11 A p-Helix Binds to a Hairpin Loop of lac Operator
- 2.12 Prediction of the Sequence of lac Operator
- 2.13 The Kinetics of lac Repressor-Operator Interaction
- 2.14 Lac Repressor Bound to O2 Acts as an Efficient Road Block
- 2.15 Auxiliary Operators Can be Disregarded in Repression
- 2.16 CAP Activates Transcription like ? CI Repressor
- 2.17 In the Pitfall of Symmetry
- 2.18 The CAP-DNA Complex: Two Propositions
- 2.19 The Adenine of Cyclic AMP Binds to DNA
- 2.20 The Structure of ß-Galactosidase
- 2.21 From Lamarck to Cairns
- 2.22 DNA Sequence Analysis: Not in this Century!
- 2.23 Protein Splicing
- 2.24 OI-Repressor-O2 Loops
- Part 3: The lac Operon, a Paradigm of Beauty and Efficiency
- 3.1 The New Perspective
- 3.2 Some Numbers and Concepts
- 3.3 Activation of the lac Promoter by CAP Protein
- 3.4 Repression of the lac Promoter by Lac Repressor
- 3.5 ß-Galactosidase, Lac Permease and Transacetylase
- 3.6 The lac System as a Tool
- 3.7 What Lesson can be Learned from the lac System?
- 3.8 Outlook
- Authors Index
- Subject Index
