Tissue Engineering Methods and Protocols
Published on 1. February 2008
XVI, 629 pages
978-1-59259-602-7 (ISBN)
Description
In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.
Reviews / Votes
"...will provide a very valuable laboratory aid for any researcher who wishes to enter the field. For the experienced cell biologist there are some gems of collected wisdom which he or she should find inspiring...a thoroughly recommended lab-bench book. . .This book should be in the library of every surgical research department. It is an excellent "how-to-do-it" work."-Journal of The Royal College of Surgeons of Edinburgh"This book, well-edited by Morgan and Yarmush, contains a nearly exhaustive series of protocols related to biomaterials, cells, composites of cells and biomaterials, and measurement techniques. The editors are known and respected in the field, and, as such, were able to attract some of the stalwart tissue engineering investigators. . . an indispensable aid to students and seasoned investigators in tissue engineering."-FEBS Letters
"...a weighty tome of over 600 pages which can really be considered a tour de force, a great achievement in both organizational and editing skills from Morgan and Yarmush, the editors. I found several of the contributions outstanding...a useful acquisition."-Cell Biology International
More details
Series
Language
English
Place of publication
Totowa
United States
Publishing group
Humana Press
Target group
Professional and scholarly
Product notice
Reflowable
Illustrations
XVI, 629 p. 139 illus.
File size
45,46 MB
ISBN-13
978-1-59259-602-7 (9781592596027)
DOI
10.1385/0896035166
Schweitzer Classification
Other editions
Additional editions
Jeffrey R. Morgan | Martin L. Yarmush
Tissue Engineering Methods and Protocols
Book
01/1998
Humana Press Inc.
€106.95
Article exhausted; check for reprint
Content
Materials.- Preparation of Collagen-Glycosaminoglycan Copolymers for Tissue Regeneration.- Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology.- Nondestructive Evaluation of Biodegradable Porous Matrices for Tissue Engineering.- Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering.- Formation of Highly Porous Polymeric Foams with Controlled Release Capability.- Magnetic-Induced Alignment of Collagen Fibrils in Tissue Equivalents.- Preparation and Use of Thermosensitive Polymers.- In Situ Photopolymerized Hydrogels for Vascular and Peritoneal Wound Healing.- Fabrication and Implantation of Gel-Filled Nerve Guidance Channels.- Small Animal Surgical and Histological Procedures for Characterizing the Performance of Tissue-Engineered Bone Grafts.- Preparation and Use of Porous Poly(?-Hydroxyester) Scaffolds for Bone Tissue Engineering.- Cells.- Quantitative Assessment of Autocrine Cell Loops.- Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy.- Preparation of Immortalized Human Chondrocyte Cell Lines.- Clinical Applications.- Isolation and In Vitro Proliferation of Chondrocytes, Tenocytes, and Ligament Cells.- Culture and Identification of Autologous Human Articular Chondrocytes for Implantation.- Organogenesis of Skeletal Muscle in Tissue Culture.- Hepatocyte Primary Cultures.- Formation and Characterization of Hepatocyte Spheroids.- Isolation and Culture of Human Endothelial Cells.- Isolation and Culture of Microvascular Endothelial Cells.- Initiation, Maintenance, and Quantification of Human Hematopoietic Cell Cultures.- Methods to Isolate, Culture, and Study Osteoblasts.- Cryopreservation of Rat Hepatocytes in a Three-Dimensional CultureConfiguration Using a Controlled-Rate Freezing Device.- Cell Material Composites.- Microencapsulation of Enzymes, Cells, and Genetically Engineered Microorganisms.- Methods for Microencapsulation with HEMA-MMA.- Micropatterning Cells in Tissue Engineering.- Methods for the Serum-Free Culture of Keratinocytes and Transplantation of Collagen-GAG-Based Skin Substitutes.- Use of Skin Equivalent Technology in a Wound Healing Model.- Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis.- Development of a Bioartificial Liver Device.- Methods for the Implantation of Liver Cells.- Isolation and Long-Term Maintenance of Adult Rat Hepatocytes in Culture.- Design and Fabrication of a Small Caliber Hybrid Arterial Bioprosthesis.- Methods for the Immunoisolation and Transplantation of Pancreatic Cells.- Methods for the Study of Nerve Cell Migration and Patterning.- Measurement Techniques.- Estimating Number and Volume of Islets Transplanted Within a Planar Immunobarrier Diffusion Chamber.- Quantitative Measurement of Cell-Cell Adhesion Under Flow Conditions.- Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation.- Measuring Receptor-Mediated Cell Adhesion Under Flow.- In Vitro and In Vivo Quantification of Adhesion Between Leukocytes and Vascular Endothelium.- Quantitative Measurement of Shear-Stress Effects on Endothelial Cells.- Quantitative Modeling of Limitations Caused by Diffusion.
