Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures
Published on 15. June 2015
36 pages
978-1-78040-752-4 (ISBN)
Description
Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP's developed.
This book is co-published with Water Research Australia.
This book is co-published with Water Research Australia.
More details
Language
English
Place of publication
London
United Kingdom
Target group
College/higher education
Professional and scholarly
File size
1,92 MB
ISBN-13
978-1-78040-752-4 (9781780407524)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Paul Monis | Nic Reid | Australian Water Quality Centre
Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures
Book
06/2015
IWA Publishing
€84.38
Shipment within 3-4 weeks
Content
- Cover
- Copyright
- Contents
- 1. PCR Detection of Cryptosporidium
- Preface and Foreword
- Health and Safety Information
- Performance Characteristics
- Scope
- Principle
- Reagents
- 18S rDNA Taqman Procedure
- gp60 SYTO9 Procedure
- Referenced Documents
- 2. PCR Detection of Ammonia Oxidising Prokaryotes
- Preface and Foreword
- Health and Safety Information
- Performance Characteristics
- Scope
- Principle
- Reagents
- DNA extraction
- PCR Procedures
- Referenced Documents
- 3. PCR Detection of Microcystis And Potential Microcystin Producing Cyanobacteria
- Preface and Foreword
- Health and Safety Information
- Performance Characteristics
- Scope
- Principle
- Reagents
- 16S rDNA Taqman Procedure
- mcyE SYTO9 Procedure
- Referenced Documents
- 4. PCR Detection of Adenovirus
- Preface and Foreword
- Health and Safety Information
- Performance Characteristics
- Scope
- Principle
- Reagents
- Hexon Taqman Procedure
- Referenced Documents
