Molecular Characterization of Autophagic Responses Part A
Academic Press
Published on 18. February 2017
598 pages
978-0-12-809795-3 (ISBN)
Description
Molecular Characterization of Autophagic Responses, Part A, presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man.
Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.
- Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses
- Written in an accessible style by renowned experts in the field
More details
Other editions
Additional editions
Lorenzo Galluzzi | Guido Kroemer | Jose Manuel Bravo-San Pedro
Molecular Characterization of Autophagic Responses Part A: Volume 587
Book
02/2017
Academic Press
€188.50
Shipment within 15-20 days
Persons
Content
1. Correlative Live Cell and Super Resolution Imaging of Autophagosome Formation2. Quantifying Autophagic Structures in Mammalian Cells Using Confocal Microscopy3. The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo4. Turnover of Lipidated LC3 and Autophagic Cargoes in Mammalian Cells5. High-Throughput Quantification of GFP-LC3+ Dots by Automated Fluorescence Microscopy6. Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses7. Production of Human ATG Proteins for Lipidation Assays8. Investigating Structure and Dynamics of Atg8 Family Proteins9. Methods for Studying Interactions Between Atg8/LC3/GABARAP and LIR-Containing Proteins10. Assessment of Posttranslational Modifications of ATG proteins11. Tagged ATG8-Coding Constructs for the In Vitro and In Vivo Assessment of ATG4 Activity12. Measurement of the Activity of the Atg4 Cysteine Proteases13. Crystallographic Characterization of ATG Proteins and Their Interacting Partners14. Dynamics of Atg5-Atg12-Atg16L1 Aggregation and Deaggregation15. Fluorescent FYVE Chimeras to Quantify PtdIns3P Synthesis During Autophagy16. Quantification of Phosphatidylinositol Phosphate Species in Purified Membranes17. Mass Assays to Quantify Bioactive PtdIns3P and PtdIns5P During Autophagic Responses18. Fluorescence-Based Assays to Analyse Phosphatidylinositol 5-Phosphate in Autophagy19. Ultrastructural Characterization of Phagophores Using Electron Tomography on Cryoimmobilized and Freeze Substituted Samples20. A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells21. In Vitro Reconstitution of Autophagosome-Lysosome Fusion22. In Vitro Reconstitution of Atg8 Conjugation and Deconjugation23. Study of ULK1 Catalytic Activity and Its Regulation24. Evaluating the mTOR Pathway in Physiological and Pharmacological Settings25. Methods to Study the BECN1 Interactome in the Course of Autophagic Responses26. In Vitro Characterization of VPS34 Lipid Kinase Inhibition by Small Molecules27. Methods to Study Lysosomal AMPK Activation28. Allosteric Modulation of AMPK Enzymatic Activity: In Vitro Characterization29. Assessing the Catalytic Activity of Transglutaminases in the Context of Autophagic Responses
