Molecular Characterization of Autophagic Responses Part B
Academic Press
Published on 22. February 2017
608 pages
978-0-12-809794-6 (ISBN)
Description
Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man.
Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.
- Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses
- Written in an accessible style by renowned experts in the field
More details
Other editions
Additional editions
Lorenzo Galluzzi | Guido Kroemer | Jose Manuel Bravo-San Pedro
Molecular Characterization of Autophagic Responses Part B: Volume 588
Book
02/2017
Academic Press
€188.50
Shipment within 15-20 days
Persons
Content
1. Renilla Luciferase-LC3 Based Reporter Assay for Measuring Autophagic Flux2. Measurement of Autolysosomal pH by Dual-Wavelength Ratio Imaging3. Long-Lived Protein Degradation During Autophagy4. Proteomic Profiling of De Novo Protein Synthesis in Starvation-Induced Autophagy Using Bioorthogonal Noncanonical Amino Acid Tagging5. Methods to Monitor and Manipulate TFEB Activity During Autophagy6. Application of CRISPR/Cas9 to Autophagy Research7. A Molecular Reporter for Monitoring Autophagic Flux in Nervous System In Vivo8. Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy9. Assessment of Glycolytic Flux and Mitochondrial Respiration in the Course of Autophagic Responses10. Methods to Assess Mitochondrial Morphology in Mammalian Cells Mounting Autophagic or Mitophagic Responses11. Monitoring Mitophagy in Mammalian Cells12. Cytofluorometric Assessment of Mitophagic Flux in Mammalian Cells and Tissues13. Automated Analysis of Fluorescence Colocalization: Application to Mitophagy14. Assays to Monitor Lysophagy15. Kinetics of Protein Aggregates Disposal by Aggrephagy16. Methods to Study Chaperone-Mediated Autophagy17. Quantitative Assay of Macroautophagy Using Pho8¿60 Assay and GFP-Cleavage Assay in Yeast18. Monitoring the Formation of Autophagosomal Precursor Structures in Yeast Saccharomyces cerevisiae19. Methods to Assess Autophagy and Chronological Aging in Yeast20. Methods to Measure Lipophagy in Yeast21. Assays to Monitor Pexophagy in Yeast22. Monitoring Autophagic Responses in Caenorhabditis elegans23. Characterization of Autophagic Responses in Drosophila melanogaster24. Methods to Study Autophagy in Zebrafish25. Biochemical Methods to Monitor Autophagic Responses in Plants26. Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants
