Methods in Cell Biology

Name: Methods in Cell Biology
Brand: Academic Press
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Academic Press

Published on 13. January 1977

421 pages

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978-0-08-085911-8 (ISBN)

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Cover
Contributors to This Volume
Contents
List of Contributors
Preface
Chapter 1. Enhancement of virus-Induced Cell Fusion by Phytohemagglutinin
I. Introduction
II. Phytohemagglutinin (PHA)
III. Virus-Induced Cell Fusion and Quantitation of Hybrid Cell Formation
IV. Enhancement of Virus-Induced Cell Fusion and Hybridization by PHA
V. Mechanism of PHA-Induced Enhancement of Cell Fusion
References
Chapter 2. Methods of Cell Fusion with Germiston Virus
I. Introduction
II. Preparation of Germiston Virus
III. Cell Fusion
IV. Comments
References
Chapter 3. Fusion of Mammalian Cells by Lipid Vesicles
I. Introduction
II. Preparation of Lipid Vesicles
III. Cell Fusion Technique
IV. Fusion and Hybridization of Mammalian Cells by Lipid Vesicles: Comparison with Virus and Chemically Induced Fusion
V. Discussion
References
Chapter 4. Lipid Vesicles as carriers for Introducing Biologically Active Materials into Cells
I. Introduction
II. Preparation of Lipid Vesicles
III. Vesicle Composition
IV. Physicochemical Properties of Lipid Vesicles
V. Incorporation of Lipid Vesicles by Cells in Vitro and in Vivo
VI. Use of Lipid Vesicles to Introduce Materials into Cells in Vitro and in Vivo
VII. Targeting of Lipid Vesicles to Specific Intracellular Compartments
VIII. ?HomingZ of Vesicles to Specific Cell Types
IX. Conclusions
References
Chapter 5. Use of Erythrocyte Ghosts for Injection of Substances into Animal Cells by Cell Fusion
I. Introduction
II. Method of Injection
III. Advantages and Limitations
IV. Possible Applications
V. An Example of the Use of This Method: Neutralization of Diphtheria Toxin in Living Cells
References
Chapter 6. The Production of Binucleate Mammalian Cell Populations
I. Introduction
II. Methods
III. Results
IV. Discussion
V. Summary
References
Chapter 7. Enucleation of Mammalian Cells in Suspension
I. Introduction
II. Enucleation in Isopycnic Suspension
III. Results
IV. Discussion
References
Chapter 8. Cell Culture with Synthetic Capillaries
I. Introduction
II. General Characteristics
III. Transport Aspects
IV. Capillary Perfusion Systems
V. Applications and Experience
VI. Future Development
References
Chapter 9. Magnesium Acetate Treatment of Glass Roller Bottles to Facilitate Cell Attachment
I. Introduction
II. Design of Equipment and Procedures for Using Roller Bottles
III. Treatment of Roller Bottles to Enhance Cell Attachment
IV. Discussion
References
Chapter 10. Use of Plastics for Suspension Culture Vessels
I. Introduction
II. Use of Plastics for Monolayer Cultures
III. Adaptation of Cell Lines to Growth in Suspension
IV. Toxicity Testing of Plastic Laboratory Ware
V. Construction of Plastic Suspension Culture Vessels
VI. Conclusions
References
Chapter 11. Determination of the Growth Rate in Human Fibroblasts in Cell Culture
I. Introduction
II. Measuring Proliferation of Individual Clones or Colonies
III. Method
IV. Application
V. Conclusion
References
Chapter 12. Cultivation of Mammalian Cell Lines in Serum-Free Chemically Defined Medium
I. Introduction
II. Materials
III. Methods
IV. Concluding Remarks on Future of Defined Medium
References
Chapter 13. Improved Synthetic Media Suitable for Tissue Culture of Various Mammalian Cells
I. Introduction
II. Composition of Chemically Defined Synthetic Media
III. Growth of Cells in Various Media
IV. Effects of Composition of Buffer and Salts in Media
Addendum
References
Chapter 14. Automatic Collection of Samples from Suspension Cell Cultures for Investigation of Prolonged Metabolic Processes
I. Introduction
II. Apparatus
III. Results and Discussion
References
Chapter 15. A Simple Device and Procedure for Successful Freezing of Cells in Liquid Nitrogen Vapor
I. Introduction
II. Materials and Methods
III. Results
IV. Discussion
V. Recommended Procedure
VI. Summary
References
Chapter 16. Long-Term Preservation of the Ehrlich Ascites Tumor
I. Introduction
II. Initial Studies with Glycerol
III. Prolonged Storage with Glycerol
IV. Stains as Viability Indicators
V. Speculations on Improving the Preservation
References
Chapter 17. Rapid Biochemical Screening of Large Numbers ofAnimal Cell Clones
I. Introduction
II. Mutagenesis and Mutation Frequency
III. Screening
IV. Closing Comments
References
Chapter 18. Use of Polyacrylamide for Cloning of Primary Tumors
I. Introduction
II. Methods
III. Discussion
References
Chapter 19. Isolation of Drug-Resistant Clones of Ehrlich Ascites Tumor Cells
I. Introduction
II. Procedure
III. Biochemical Characterization
IV. Discussion
References
Chapter 20. Separation of Clonogenic Cells from Stationary Phase Cultures and a Murine Fibrosarcoma by Density-Gradient Centrifugation
I. Introduction
II. Materials and Methods
III. Results
IV. Discussion
References
Chapter 21. Rapid Screening Assay for Revertants of Murine Sarcoma Virus- Transformed Cells
I. Introduction
II. Methods
III. Sensitivity of Assay
IV. Reliability of Assay
V. Application of Rapid Screening Assay
References
Chapter 22. Selective Techniques for the Isolation of Morphological Revertants of Sarcoma Virus-Transformed Cells
I. Introduction
II. Methods of Preparation of Transformed Cells for Selection of Revertants
III. In Vitro Biological Properties of Morphological Revertants
IV. In Vivo Biological Properties of Morphological Revertants
V. Conclusions
References
Chapter 23. Selection, Screening, and Isolation of Temperature-Sensitive Mutants of Avian Sarcoma Viruses
I. Introduction
II. Theoretical Considerations in ts Mutant Isolation
III. Methods Applicable to the Selection and Screening of ASV ts Mutants
IV. Discussion
References
Chapter 24. Induction and Isolation of Cold-Sensitive Lines of Chinese Hamster Cells
I. Introduction
II. Cell Line
III. Choice of Temperatures
IV. Mutagenesis
V. Selection
VI. Isolation, Testing, and Purifying of Colonies
VII. General Characteristics of Cold-Sensitive Lines
VIII. Conclusions
References
Chapter 25. Maintenance of Perpetual Synchrony in HeLa S3 Culture: Theoretical and Empirical Approaches
I. Introduction
II. A Simplistic Model of Induced Synchrony
III. Choice of a Reversible Inhibitor
IV. Result of Resynchronization Procedure
V. Conclusions
References
Chapter 26. A Method for Synchronization of Animal Cells in Culture
I. Introduction
II. Cell Culture and Media
III. Preparation of Synchronous Cell Populations
IV. Methods for Characterization of Synchronous Cultures
V. Characterization of the Synchronization
VI. Concluding Remarks
References
Chapter 27. Hypertonicity and the Synchronization of Mammalian Cells in Mitosis
I. Objectives and Rationale
II. Techniques
III. Ions, Macromolecular Synthesis, and Cell Division
IV. Comment
References
Chapter 28. Selection of Synchronized Populations of HeLa Cells
I. Introduction and Review of Synchronization Methodology
II. Methods and Materials
III. Results and Discussion
References
Chapter 29. The Preparation and Characterization of Intact Isolated Parenchymal Cells from Rat Liver
I. Introduction
II. Preparation of Cells
III. Characterization of Isolated Cells
IV. Culture of Isolated Cells
V. Potential Errors in Evaluating Data
VI. Conclusion
References
Chapter 30. Selective Cultivation of Mammalian Epithelial Cells
I. Introduction
II. Primary Culture Method
III. Epithelial Cell Strains from Mouse Tissues
IV. Epithelial Cell Strains from Human Tissues
V. Discussion
References
Chapter 31. Primavy and Long-Term Culture of Adult Rat Liver Epithelial Cells
I. Introduction
II. Methods
III. Results
IV. Discussion
References
Chapter 32. Isolation of Rat Peritoneal Mast Cells in High Yield and Purity
I. Introduction
II. Procedures
III. Characteristics of Purified Mast Cell Preparation
IV. Discussion
References
Chapter 33. Monocyte Preparation from Blood
I. Introduction
II. The Identification of Monocytes
III. Methods of Isolating Monocytes
IV. Discussion and Some Unsolved Problems Concerning This Method of Separation
References
Chapter 34. Differentiation and Proliferation of Hemopoietic Cells in Culture
I. Introduction
II. Stem Cell Assay
III. CFUc Assay
IV. Liquid Cultures
References
Index
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Methods in Cell Biology

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