Methods in Cell Biology
Academic Press
Published on 24. May 1976
326 pages
978-0-08-085910-1 (ISBN)
Description
Methods in Cell Biology
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D.Marshall Prescott
Methods in Cell Biology: v.13
Book
04/1976
Academic Press
€136.40
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Content
- Cover
- Contributors to This Volume
- Contents
- List of Contributors
- Preface
- Chapter 1. Large-Scale Enucleation of Mammalian Cells
- I. Introduction
- II. Cells and Materials
- III. Techniques
- References
- Chapter 2. Reconstruction of Cultured Mammalian Cells from Nuclear and Cytoplasmic Parts
- I. Introduction
- II. Preparation of Components for Cell Reconstruction
- III. Criteria for Determination of Reconstructed Cells
- IV. Protocol for Cell Reconstruction
- V. Conclusions
- References
- Chapter 3. Recording of Clonal Growth of Mammalian Cells through Many Generations
- I. Introduction
- II. Culture Medium
- III. Preparation of the Cells
- IV. Microphotographic Recording of Clonal Growth
- V. Clonal Growth
- VI. Applications
- References
- Chapter 4. Preparation of Isolated Rat Liver Cells
- I. Introduction
- II. Quality Evaluation
- III. Nonenzymatic Methods for Liver Cell Preparation
- IV. Enzymatic Treatment of Liver Slices
- V. Collagenase Perfusion
- VI. Purification of Parenchymal Cells
- VII. Incubation of Cell Suspensions
- VIII. Properties of Isolated Parenchymal Cells
- IX. In Vitro-Culture of Parenchymal Liver Cells
- X. Preparation of Nonparenchymal Liver Cells
- References
- Chapter 5. Isolation and Subfractionation of Mammalian Sperm Heads and Tails
- I. Introduction
- II. Isolation of Sperm Heads and Tails
- III. Subfractionation of the Sperm Head
- IV. Subfractionation of the Tail
- References
- Chapter 6. On the Measurement of Tritium in DNA and Its Applications to the Assay of DNA Polymerase Activity
- I. Introduction
- II. Evaluation of Methods for Recipitation, Collection, and Counting of Tritium-Labeled DNA
- III. Determination of Optimal Conditions for Collection and Counting of DNA Polymerase Assay Products
- IV. Selection of Methods for a Particular System
- References
- Chapter 7. The Radioiodination of RNA and DNA to High Specific Activities
- I. Introduction
- II. Radioiodine
- III. Commerford's Reaction: General Description
- IV. Equipment Needs
- V. Radioiodination Reaction
- VI. Comments and Discussion
- References
- Chapter 8. Density Labeling of Proteins
- I. Introduction
- II. Density Labeling
- III. Equilibrium Density Gradient Sedimentation
- IV. Ultracentrifugation
- V. Use of Density Markers
- VI. Combination with Other Biochemical Methods
- VII. Conclusions
- References
- Chapter 9. Techniques for the Autoradiography of Diffusible Compounds
- I. Introduction
- II. Dry-Mount Autoradiography
- III. Thaw-Mount Autoradiography
- IV. Smear-Mount Autoradiography
- V. Touch-Mount Autoradiography
- References
- C$Chapter 10. Characterization of Estrogen-Binding Proteins in Sex Steroid Target Cells Growing in Long-Term Culture
- 1. Introduction
- II. Materials and Methods
- Ill. Discussion
- References
- Chapter 11. Long-Term Amphibian Organ Culture
- I. Introduction
- II. Animals
- III. Methods
- IV. Results
- V. Concluding Remarks
- References
- Chapter 12. A Method for the Mass Culturing of Large Free-Living Amebas
- I. Introduction
- II. The Basic Ameba Culture Procedure
- III. The Culturing and Harvesting of the Food Organism Tetrahymena pyriformis
- IV. The Cleaning and Harvesting of Ameba Cultures
- V. Concluding Remarks
- References
- Chapter 13. Induction and Isolation of Mutants in Tetrahymena
- I. Introduction
- II. Elements of Tetrahymena Genetics
- III. Strains
- IV. Media
- V. Routine Methods
- VI. Calibration and Suggested Doses of Mutagens
- VII. Strategies and Protocols for Mutant Isolation
- VIII. Genetic Analysis of the Mutants
- IX. Additional Information for Nongeneticists
- References
- Chapter 14. Isolation of Nuclei from Protozoa and Algae
- I. Introduction
- II. Monitoring the Reparation
- III. Tetrahymena
- IV. Paramecium
- V. Blepharisma
- VI. Didinium
- VII. Spirostomum
- VIII. Amoeba
- IX. Euglena
- X. Algae
- References
- Index
- Contents of Previous Volumes
