Methods in Cell Biology
Academic Press
Published on 12. November 1975
451 pages
978-0-08-085907-1 (ISBN)
Description
Methods in Cell Biology
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D.Marshall Prescott
Methods in Cell Biology: v. 10
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09/1975
Academic Press
€136.40
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Content
- Cover
- Contributors to This Volume
- Contents
- List of Contributors
- Preface
- Contents of Previous Volumes
- Chapter 1. Nucleic Acid Hybridization to the DNA of Cytological Preparations
- Introduction
- I. Procedure for in Situ Hybridization
- II. Equipment and Reagents
- III. Radioactive Nucleic Acids for Hybridization
- IV. Conditions of Reaction
- V. General Comments
- References
- Chapter 2. Microanalysis of RNA from Defined Cellular Components
- I. Introduction
- II. Experimental Material
- III. Labeling Conditions
- IV. Preparation and Fixation
- V. Microdissection
- VI. Microextraction
- VII. Microelectrophoresis in Gels
- VIII. Microhybridization
- IX. Combination of Micro- and Macrotechniques
- X. Applications
- References
- Chapter 3. Staining of RNA after Polyacrylamide Gel Electrophoresis
- I. Introduction
- II. Action of Fixatives and Stains on RNA
- III. Procedure for RNA Staining
- IV. Evaluation of the Stained RNA Pattern
- V. Other Methods of RNA Detection
- VI. Concluding Remarks
- References
- Chapter 4. Isolation and Fractionation of Mouse Liver Nuclei
- I. Fine Structure of Nuclei Isolated in Various Ionic Media
- II. Fractionation of Mouse Liver Nuclei
- III. Purification of Nuclear Membranes from Mouse Liver Nuclei
- IV. Conclusions
- References
- Chapter 5. The Separation of Cells and Subcellular Particles by Colloidal Silica Density Gradient Centrifugation
- I. Introduction
- II. Materials and Methods
- III. Applications
- IV. Conclusions
- References
- Chapter 6. Isolation of Subcellular Membrane Components from Tetrahymena
- I. Introduction
- II. Systematic Isolation Methods for Various Subcellular Membrane Components
- III. Nonsystematic Isolation Methods for Various Subcellular Membrane Components
- IV. Membrane Lipid Composition of Various Isolated Subcellular Components
- References
- Chapter 7. Isolation and Experimental Manipulation of Polytene Nuclei in Drosophila
- I. Introduction
- II. Isolation of Nuclei and Nucleoli
- III. Nuclear Purification in Sucrose Gradients
- IV. Cytological Analysis of Isolated Nuclei
- V. Manipulation and Incubation of Polytene Nuclei
- VI. Related Studies
- References
- Chapter 8. Methods for Microsurgical Production of Mammalian Somatic Cell Hybrids and Their Analysis and Cloning
- I. Introduction
- II. Cell Culture
- III. Cell Fusion
- IV. Cell Isolation
- V. Analyzing and Cloning Hybrid Cells
- VI. Additional Comments
- References
- Chapter 9. Automated Cell Cycle Analysis
- I. Introduction
- II. Cell Cycle Analyzer
- III. Optimizing Selection Conditions
- IV. Calibrating the Cell Cycle
- V. Limitations and Prospects
- References
- Chapter 10. Selection of Synchronous Cell Populations from Ehrlich Ascites Tumor Cells by Zonal Centrifugation
- I. Introduction
- II. Materials
- III. Zonal Centrifugation Run
- IV. Results
- V. Possible Applications
- References
- Chapter 11. Methods with Insect Cells in Suspension Culture
- I. Introduction
- II. Adaptation of A. albopictus Cells to Desired Growth Conditions
- III. Characteristics of the Cell Line
- IV. Cell Fractionation and Other Procedures
- V. Conclusion
- References
- Chapter 12. Methods with Insect Cells in Suspension Culture
- I. Introduction
- II. Adaptation of the Line to a New Medium and to Suspension Growth
- III. Characteristics of the Cells
- IV. Cell Fractionation
- V. Summary and Conclusions
- References
- Chapter 13. Mutagenesis in Cultured Mammalian Cells
- I. Introduction
- II. Conditions for Experiments on Mutagenesis
- III. Spontaneous Mutagenesis
- IV. Induced Mutagenesis
- References
- Chapter 14. Measurement of Protein Turnover in Animal Cells
- I. Introduction
- II. Methods for the Measurement of ks
- III. Measurement of kd
- IV. Conclusions
- References
- Chapter 15. Detection of Mycoplasma Contamination in Cultured Cells: Comparison of Biochemical, Morphological, and Microbiological Techniques
- I. Introduction
- II. Microbiological Culture
- III. Morphological Techniques
- IV. Biochemical Techniques
- V. Comparative Studies of Biochemical, Morphological, and Microbiological Techniques for the Detection of Mycoplasma Contamination
- VI. Sensitivities of Biochemical, Morphological, and Microbiological Techniques for Mycoplasma Detection
- VII. Discussion
- References
- Chapter 16. A Simple Biochemical Technique for the Detection of Mycoplasma Contamination of Cultured Cells
- I. Introduction
- II. Alterations in Exogenous Uridine and Uracil Incorporation into RNA in Mycoplasma-Infected Cells
- III. Measurement of the Ratio of Uridine-3H (Urd) to Uracil-3H (U) Incorporated into Cellular RNA
- IV. Evaluation of Results
- V. Effect of Incubation Time, Cell Concentration, Antibiotics, and Serum Batch, Age, and Concentration on Ura/U Ratios
- VI. Comparison of Parallel Single-Isotope and Double-Isotope Labeling for Urd/U Determinations
- VII. Discussion
- References
- Chapter 17. Purity and Stability of Radiochemical Tracers in Autoradiography
- I. Introduction
- II. Design of Tracer Experiments Using Autoradiography
- III. Concepts of Purity
- IV. Self-Decomposition of Radiochemicals-Obserations and Control
- V. Importance of the Specificity of Labeling
- VI. Factors Associated with the Autoradiographic Techniques
- VII. Concluding Remarks
- References
- Chapter 18. 125I in Molecular Hybridization Experiments
- I. Introduction
- II. Iodination Methodology
- III. Applications of 125I-Labeled Nucleic Acids
- IV. Discussion
- References
- Chapter 19. Radioiodine Labeling of Ribopolymers for Special Applications in Biology
- I. Introduction
- II. Special Considerations in Preliminary Preparation of the RNA
- III. Radioiodination of Polynucleotides
- IV. Applications of Iodine-Labeled RNA and Nucleotides
- References
- Chapter 20. Autoradiographic Analysis of Tritium on Polyacrylamide Gel
- I. Introduction
- II. Procedure
- References
- Chapter 21. A Radioautographic Method for Cell Affinity Labeling with Estrogens and Catecholamines
- I. Introduction
- II. Procedure
- III. Results
- IV. Concluding Remarks
- References
- Index
- Cumulative Subject Index Volumes I-X
