Methods in Cell Biology
Academic Press
Published on 25. July 1975
396 pages
978-0-08-085906-4 (ISBN)
Description
Methods in Cell Biology
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D.Marshall Prescott
Methods in Cell Biology: v. 9
Book
10/1975
Academic Press
€73.79
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Content
- Cover
- Contributors to This Volume
- Contents
- List of Contributors
- Preface
- Contents of Previous Volumes
- Chapter 1. Preparation of Large Quantities of Pure Bovine Lymphocytes and a Monolayer Technique for Lymphocyte Cultivation
- I. Introduction
- II. Preparation of Bovine Lymphocytes
- III. Cultivation of Bovine Small Lymphocytes in a Monolayer
- References
- Chapter 2. Methods to Culture Diploid Fibroblasts on a Large Scale
- I. Introduction
- II. Establishing a Human Fibroblast Culture
- Ill. An Automated Method for Growing Cells on Glass Beads (Perlacell)
- IV. Other Methods for Mass-Scale Culturing of Fibroblasts
- References
- Chapter 3. Partition of Cells in Two-Polymer Aqueous Phases: A Method for Separating Cells and for Obtaining Information on Their Surface Properties
- I. Introduction
- II. Methods
- III. Results
- IV. Concluding Remarks
- References
- Chapter 4. Synchronization of Cell Division in Vivo through the Combined Use of Cytosine Arabinoside and Colcemid
- I. Introduction
- II. Materials and Methods
- III. Results
- IV. Discussion
- References
- Chapter 5. The Accumulation and Selective Detachment of Mitotic Cells
- I. Introduction
- II. Preparation of Mitotic Cells
- III. Colcemid and Mitotic Arrest
- IV. Cell Cycle AnaIysis
- V. Application to Other Cell Lines
- VI. Human Diploid Cells
- VII. Discussion
- References
- Chapter 6. Use of the Mitotic Selection Procedure for Cell Cycle Analysis: Emphasis on Radiation-Induced Mitotic Delay
- I. Introduction
- II. Materials and Methods
- III. Results
- IV. Discussion
- References
- Chapter 7. Evaluation of S Phase Synchronization by Analysis of DNA Replication in 5-Bromodeoxyuridine
- I. Introduction
- II. Methods
- III. Results
- IV. Discussion
- References
- Chapter 8. Application of Precursors Adsorbed on Activated Charcoal for Labeling of Mammalian DNA in Vivo
- I. Introduction
- II. Preparation of Adsorbed Precursors
- III. Kinetics of Labeling of Mammalian DNA after a Single Injection of Adsorbed Thymidine-14C
- IV. Applications of the Technique
- V. Concluding Remarks
- References
- Chapter 9. Growth of Functional Glial Cells in a Serumless Medium
- I. Background Leading to Current Studies
- II. Composition, Preparation, and Storage of Synthetic Medium
- III. Adaptation and Care of Cells in Serumless Medium
- IV. Functional Characteristics of Adapted Cells
- V. Attempts to Grow Other Cells with Differentiated Characteristics in Serumless Medium
- VI. Significance of Studies and Future Directions
- References
- Chapter 10. Miniature Tissue Culture Technique with a Modified (Bottomless) Plastic Microplate
- I. Introduction
- II. Preparation of the Microplates
- III. Assembly of the Microplate for Use
- IV. Tissue Culture Procedures
- V. Use of the Plates
- VI. Removal of Slides
- VII. Staining of Slides
- VIII. Recycling the Plates
- References
- Chapter 11. Agar Plate Culture and Lederberg-Style Replica Plating of Mammalian Cells
- I. Introduction
- II. Agar Plate Culture
- III. Lederberg-Style Replica Plating of Mammalian Cells
- IV. Application of Agar Plate Culture and Replica Plating to Mammalian Cell Genetics
- V. Concluding Remarks
- References
- Chapter 12. Methods and Applications of Flow Systems for Analysis and Sorting of Mammalian Cells
- I. Introduction
- II. Operational Features of the Cell Analysis and Sorting System
- III. Light-Scattering Properties of Cells and Cell Models
- IV. Cell Preparative Procedures for Flow-System Analysis
- V. Methods for Staining Cells in Liquid Suspension
- VI. DNA Content Measurements of Diploid and Heteroploid Cells
- VII. CellCycle Analysis
- VIII. Analyses Employing Immunofluorescence Techniques
- IX. Determination of the Cell Surface Density Binding of Concanavalin A
- X. Applications of Cell-Sorting Techniques
- XI. Potential Applications for Fluorescent Enzyme Assay
- XII. Summary and Conclusions
- Appendix A: Cell Dispersal
- Appendix B: Acriflavin-Feulgen Procedure
- Appendix C: Ethidium Bromide (EB) and Propidium Iodide (PI) Protocols
- References
- Chapter 13. Purification of Surface Membranes from Rat Brain Cells
- I. The Neuronal Surface
- II. Isolation Methods
- III. Composition of Brain Plasma Membranes
- IV. Synthesis of Proteins and Glycoproteins of Surface Membranes
- References
- Chapter 14. The Plasma Membrane of KB Cells
- lsolation and Properties
- I. Introduction
- II. Culture Conditions and Various Media
- III. Enzymic Assays
- IV. Analytical Methods
- V. Isolation of Plasma Membranes by the Latex Bead Method
- VI. Isolation of Plasma Membranes by the ZnCl2 Method
- VII. Isolation of Mitochondria and Microsomes from KB Cells
- VlII. Electron Microscopy
- IX. Kinetics of Bead Uptake
- X. Morphological Studies
- XI. Biochemical Studies
- XII. Kinetics of Transport of a-Aminoisobutyric Acid (AIB) in KB Cells After Ingestion of Latex Beads
- XIII. Purity of the Plasma Membranes
- XIV. Validity of the Latex Bead Method
- XV. Damage of the Plasma Membrane by Tris Buffer
- XVI. Applicability of the Latex Bead Method to Other Cells
- References
- Chapter 15. The Isolation of Nuclei from Paramecium aurelia
- I. Introduction
- II. Isolation of Macronuclei
- III. Isolation of Micronuclei
- IV. Perspectives
- References
- Chapter 16. Isolation of Micro- and Macronuclei of Tetrahymena pyriformiss
- I. Introduction
- II. Cell Culture
- Ill. Isolation of Nuclei
- IV. Yields and Purity of Isolated Nuclei
- V. Structural, Chemical, and Metabolic Studies of Isolated Nuclei
- References
- Chapter 17. Manipulations with Tetrahymena pyriformis on Solid Medium
- I. Introduction
- II. Growth and Cloning of Tetrahymena pyriformis on Solid Medium
- III. Summary and Conclusions
- References
- Chapter 18. The Isolation of Nuclei with Citric Acid and the Analysis of Proteins by Two-Dimensional Polyacrylamide Gel Electrophoresis
- I. Introduction
- II. Methods
- III. Morphology of Sucrose and Citric Acid Nuclei
- IV. Composition of Sucrose and Citric Acid Nuclei
- V. Two-Dimensional Electrophoretic Analysis of Nuclear Proteins from Citric Acid Nuclei
- VI. Isolation of Nucleoli
- VII. Summary
- References
- Chapter 19. Isolation and Manipulation of Salivary Gland Nuclei and Chromosomes
- I. Introduction
- II. Material
- III. Method
- IV. Dependence of Chromosome Structure on Ionic Environment
- References
- Index
