Methods in Cell Biology
Academic Press
Published on 27. August 1974
463 pages
978-0-08-085905-7 (ISBN)
Description
Methods in Cell Biology
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D.Marshall Prescott
Methods in Cell Biology: v. 8
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08/1974
Academic Press
€136.40
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Content
- Cover
- Contributors to This Volume
- Contents
- List of Contributors
- Preface
- Contents of Previous Volumes
- Chapter 1. Methods for Selecting and Studying Temperature-Sensitive Mutants of BHK-21 Cells
- I. Introduction
- II. Choice of Cell Lines and Temperature
- III. Mutagenesis
- IV. Selection
- V. Isolation of Surviving Cells and Testing for ts Phenotype
- VI. Frequency and General Behavior of the ts BHK Mutants
- VII. Complementation Tests and Methods to Test Dominance or Recessiveness of the ts Mutations
- VIII. Nature of the ts Mutants
- IX. Conclusions
- References
- Chapter 2. Induction and Isolation of Auxotrophic Mutants in Mammalian Cells
- I. Introduction
- II. Procedures
- III. Special Techniques, Equipment, and Reagents
- IV. General Discussion
- References
- Chapter 3. Isolation of Temperature-Sensitive Mutants of Mammalian Cells
- I. Introduction
- II. Theoretical Considerations
- III. Methods
- IV. Discussion
- References
- Chapter 4. Preparation and Use of Replicate Mammalian Cell Cultures
- I. Introduction
- II. Replicate Culture Planting Device
- III. Preparation and Use of Replicate Mammalian Cell Cultures
- IV. Problems, and Some Suggestions
- V. Quantitation of Results
- Appendix: Standardization of Hemacytometer and Coulter Counting Methods
- References
- Chapter 5. Methods for Obtaining Revertants of Transformed cells
- I. Introduction
- II. Isolation of Revertants from Transformed Cell Lines
- III. Properties of Revertant Cells
- IV. Conclusion
- References
- Chapter 6. Monolayer Mass Culture on Disposable Plastic Spirals
- I. Introduction
- II. Apparatus
- III. Method
- IV. Harvesting
- V. Uses
- References
- Chapter 7. A Method for Cloning Anchorage-Dependent Cells in Agarose
- I. Introduction
- II. Procedure
- III. Discussion
- References
- Chapter 8. Repetitive Synchronization of Human Lymphoblast Cultures with Excess Thymidine
- I. Introduction
- II. Methods
- III. Characterization of the Synchronization
- IV. Concluding Remarks
- References
- Chapter 9. Uses of Enucleated Cells
- I. Introduction
- II. The Technology of Enucleation
- III. Uses and Properties of Enucleated Cells
- IV. Summary
- References
- Chapter 10. Enucleation of Somatic Cells with Cytochalasin B
- I. Introduction
- II. Enucleation of Somatic Cells Attached to the Inner Surface of Ultracentrifuge Nitrocellulose Tubes
- III. Enucleation of Somatic Cells on Plastic Coverslips
- IV. Results and Discussion
- References
- Chapter 11. Isolation of Mammalian Heterochromatin and Euchromatin
- I. Introduction
- II. Isolation of Nuclei
- III. Isolation of Heterochromatin and Euchromatin
- IV. Conclusion
- References
- Chapter 12. Measurements of Mammalian Cellular DNA and Its Localization in Chromosomes
- I. Introduction
- II. Banding Techniques
- III. Flow Microfluorometric Analysis
- IV. Applications of Methods to Cells in Culture
- V. Summary
- References
- Chapter 13. Large-Scale Isolation of Nuclear Membranes from Bovine Liver
- I. Introduction
- II. Large-Scale Isolation of Nuclei from Bovine Liver
- III. Isolation of Nuclear Membranes
- IV. Biochemical Applications
- References
- Chapter 14. A Simplified Method for the Detection of Mycoplasma
- I. Detection of Mycoplasma Contamination: Introduction and Background
- II. Uridine Phosphorylase Assay: Facilities, Equipment, Chemicals, and Stock Solutions
- III. Uridine Phosphorylase Assay: Description of Procedure
- IV. Levels of Uridine Phosphorylase Activity in Various Mycoplasma and Cell Types
- V. Discussion
- References
- Chapter 15. The Ultra-Low Temperature Autoradiography of Water and Its Solutes
- I. Introduction
- II. Sample Embedding and Freezing
- III. Sectioning, Preparation of the Sandwich, and Exposure
- IV. Development, Mounting, and Bleaching
- V. Analysis of Autoradiographs
- VI. Experimental Examples
- References
- Chapter 16. Quantitative Light Microscopic Autoradiography
- I. Introduction
- II. Materials and Procedures
- III. Results and Discussion
- IV. Calculations
- Appendix: Derivation of Specific Activity Equations
- References
- Chapter 17. Ionic Coupling between Nonexcitable Cells in Ctrlture
- I. Introduction
- II. Technical Equipment
- III. Determination of Cell Membrane and Junctional Membrane Resistance
- IV. Measurements of Ionic Coupling in Different Cells
- V. Conclusion
- References
- Chapter 18. Methods in the Cellular and Molecular Biology of Paramecium
- I. Cellular Biology
- II. Molecular Biology
- III. Perspectives
- References
- Chapter 19. Methods of Cell Transformation by Tumor Viruses
- I. Introduction
- II. Materials and General Methods
- III. A Brief Survey of Tumor Viruses
- IV. Neoplastic Transformation
- V. Cells in Culture
- VI. General Properties of Transformed Cells
- VII. Specific Properties of Transformed Cells
- VIII. Transformation by Papova Viruses
- IX. Transformation by Other DNA Viruses
- X. Transformation by RNA Viruses
- XI. Viral and Cellular Factors in the Transformed Phenotype
- References
- Index
- Author Index
- Subject Index
