Methods in Cell Biology
Academic Press
Published on 11. March 1974
491 pages
978-0-08-085904-0 (ISBN)
Description
Methods in Cell Biology
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D.Marshall Prescott
Methods in Cell Biology: v. 7
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01/1974
Academic Press
€136.40
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Content
- Cover
- Contributors to This Volume
- Contents
- List of Contributors
- Preface
- Contents of Previous Volumes
- Chapter 1. The Isolation of RNA from Mammalian Cells
- I. Introduction
- II. The Isolation of Subcellular Components
- III. The Extraction of RNA
- IV. RNA Purification Procedures
- V. The Isolation of Messenger RNA
- References
- Chapter 2. Preparation of RNA from Animal Cells
- I. Introduction
- II. Labeling of RNA with Radioactive Precursors
- III. Preparation of Rapidly Labeled Nuclear RNA
- IV. Preparation of Ribosomal RNA
- V. Preparation of Messenger RNA
- VI. Preparation of Transfer RNA
- VII. Preparation of Mitochondrial RNA
- VIII. Concluding Remarks
- References
- Chapter 3. Detection and Utilization of Poly(A) Sequennces in Messenger RNA
- I. Introduction
- II. Detection and Characterization
- References
- Chapter 4. Recent Advances in the Preparation of Mammalian Ribosomes and Analysis of Their Protein Composition
- I. Introduction
- II. Preparation of Ribosomes
- III. Preparation of Ribosomal Subunits
- IV. Preparation of Total Protein from Ribosomes and Ribosomal Subunits
- V. Analysis of Ribosomal Proteins
- VI. Molecular Weight Distribution and Number of Ribosomal Proteins
- VII. Purified Proteins
- VIII. Heterogeneity of Ribosomal Proteins
- IX. Methods for Correlating Structure and Function
- X. Membrane-Bound Ribosomes
- XI. Phosphorylation of Ribosomal Proteins
- XII. Translation of Specific Species of Messenger RNA into Proteins of Proven Structure
- XIII. Concluding Remarks
- References
- Chapter 5. Methods for the Extraction and Purification of Deoxyribonucleic Acids from Eukaryote Cells
- I. Introduction
- II. Physical-Chemical Characteristics of DNA
- III. General Methodology
- IV. Concluding Remarks
- V. Glossary of Reagents
- References
- Chapter 6. Electron Microscopic Visualization of DNA in Association with Cellular Components
- I. Introduction
- II. Isolation of DNA-Protein Complexes
- III. Electron Microscopy
- IV. Direct Visualization of Defined Complexes
- V. Phage-Membrane Association: A Combination of Two Methods
- VI. Eukaryote Chromatin Fibers
- References
- Chapter 7. Autoradiography of Individual DNA Molecules
- I. Introduction
- II. Labeling of DNA
- III. Spreading of DNA
- IV. Autoradiography of DNA
- V. Examples of Autoradiographs
- References
- Chapter 8. HeLa Cell Plasma Membranes
- I. Introduction
- II. Preparation of HeLa Cell Plasma Membranes
- III. Uses of the Method
- IV. Discussion
- References
- Addendum
- Chapter 9. Mass Enucleation of Cultured Animal Cells
- I. Introduction
- II. Cells
- III. Manufacture of Plastic Coverslips
- IV. Enucleation
- V. Viability of Enucleated Cells
- VI. Virus Activities in Enucleated Cells
- VII. Viability of Nuclei Removed by Centrifugation
- References
- Chapter 10. The Production of Mass Populations of Anucleate Cytoplasms
- I. Introduction
- II. Preparation of Lucite Plates
- III. Plating of Cells
- IV. Enucleation
- V. Preparation of Large Numbers of Anucleate Cytoplasms
- VI. Conclusion
- References
- Chapter 11. Anucleate Mammalian Cells: Applications in Cell Biology and Virology
- I. Introduction
- II. Enucleation of Mammalian Cells by Cytochalasin B
- III. Fusion of Anucleate and Nucleated Cells
- IV. The Transfer of Isolated Nuclei to Anucleate Cells
- V. Experimental Applications of Anucleate Mammalian Cells
- VI. Conclusions
- References
- Chapter 12. Fusion of Somatic and Gametic Cells with Lysolecithin
- I. Introduction
- II. Preparation of Lysolecithin Solution
- III. Cytotoxicity of Lysolecithin Solution
- IV. Lysolecithin-Induced Fusion
- V. Quantification Procedures
- VI. Discussion
- References
- Chapter 13. The Isolation and Replica Plating of Cell Clones
- I. Introduction
- II. The Incubation Matrix
- III. Seeding for the Isolation of Single Clones
- IV. Replica Plating
- V. Capacities of the Technique
- References
- Chapter 14. Selection Synchronization by Velocity Sedimentation Separation of Mouse Fibroblast Cells Grown in Suspension Culture
- I. Introduction
- II. Principle of Velocity Sedimentation Separation
- III. Preparation of the Gradient
- IV. Separation of Cells by Velocity Sedimentation
- V. Size of Separated Cells
- VI. Synchronous Growth of Separated Cells
- VII. Estimation of the Degree of Synchrony
- VIII. Scaling-Up Procedures
- References
- Chapter 15. Methods for Micromanipulation of Human Somatic Cells in Culture
- I. Introduction
- II. Cell Culture
- III. Equipment for Making and Controlling Microtools
- IV. Micromanipulation
- V. Photography
- VI. Concluding Remarks
- References
- Chapter 16. Tissue Culture of Avian Hematopoietic Cells
- I. Introduction
- II. Materials
- III. Procedures
- Appendix
- References
- Chapter 17. Measurement of Growth and Rates of Incorporation of Radioactive Precursors into Macromolecules of Cultured Cells
- I. Introduction
- II Culture Growth Conditions
- III. Methods for Cell Counting
- IV. Measurement of Precursor Incorporation
- V. Problems Involved in the Interpretation of Results
- References
- Chapter 18. The Measurement of Radioactive Precursor Incorporation into Small Monolayer Cultures
- I. Introduction
- II. Methods
- III. Development of Method
- IV. Applications and Modifications
- V. Summary
- References
- Chapter 19. Analysis of Nucleotide Pools in Animal Cells
- I. Introduction
- II. General Properties of Pools
- III. Nucleotide Metabolism
- IV. Transport of Precursors
- V. Isotopic Methods
- VI. Direct Chemical Methods for Pool Analysis
- VII. Miscellaneous
- References
- Index
- Author Index
- Subject Index
