Methods in Cell Biology
Academic Press
Published on 19. October 1973
404 pages
978-0-08-085903-3 (ISBN)
Description
Methods in Cell Biology
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D.Marshall Prescott
Methods in Cell Biology: v. 6
Book
10/1973
Academic Press
€136.40
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Content
- Cover
- Contents
- List of Contributors
- Preface
- Contents of Previous Volumes
- Chapter 1. Cultivation of Cells in Protein-and Lipid-Free Synthetic Media
- I. Significance of Culturing Cells in Synthetic Media
- II. Composition of Chemically Defined Synthetic Media
- III. Cell Lines Serially Grown in Synthetic Media
- IV. Initiation of Cell Growth in Synthetic Media
- V. Biomorphological Changes Induced in Cells by Transfer to Synthetic Media
- VI. Tumorigenicity of Cells Grown in Synthetic Media
- VII. Sensitivity of Cells Grown in Synthetic Media to Light Irradiation, Chemical Carcinogen Treatment, and Deep Freezing
- VIII. Factors Presumably Responsible for the Failure of Cultivation of Cells in Synthetic Media
- References
- Chapter 2. Preparation of Synchronous Cell Cultures from Early Interphase Cells Obtained by Sucrose Gradient Centrifugation
- I. Introduction
- II. Requirements to Be Satisfied by the Method
- III. Cell Line and Culture Techniques
- IV. Preparation of Partially Synchronous Cell Populations
- V. Methods Used for Characterization of Synchronous Cultures
- VI. Characteristics of Synchronous Cultures
- VII. Experimental Factors Affecting the Degree of Synchrony
- VIII. Discussion
- References
- Chapter 3. Production and Characterization of Mammalian Cells Reversibly Arrested in G1 by Growth in Isoleucine-Deficient Medium
- I. Introduction
- II. Culture Conditions Affecting Induction of G1 Arrest In Isoleucine-Deficient Medium
- III. Characterization of Cells in Isoleucine-Deficient Medium
- IV. Utilization of Cells Reversibly Arrested in G1
- V. Summary and Conclusions
- References
- Chapter 4. A Method for Measuring Cell Cycle Phases in Suspension Cultures
- I. Introduction
- II. Methods
- III. Results
- IV. Concluding Remarks
- References
- Chapter 5. A Replica Plating Method of Cultured Mammalian Cells
- I. Introduction
- II. Procedure for Replica Plating of Cultured Mouse 3T6 Cells and HeLa S3 Cells
- III. Detection and Isolation of Nutritionally Deficient Mutants from the Original Chinese Hamster Hai Cells by a Replica Plating Method
- IV. Concluding Remarks
- References
- Chapter 6. Cell Culture Contaminants
- I. Introduction
- II. Mycoplasma Contamination of Cultured Cells
- III. Virus Contamination of Cultured Cells
- IV. Bacteria and Fungi Contamination of Cultured Cells
- V. Parasite Contamination of Cultured Cells
- VI. Cell Contamination by Cultured Cells
- VII. Summary
- References
- Chapter 7. Isolation of Mutants of Cultured Mammalian Cells
- I. Introduction
- II. Culture Requirements
- III. Isolation of Drug-Resistant Mutants
- IV. Isolation of Auxotrophic and Conditional Lethal Mutants by Indirect Selection
- V. Mutant Frequency and the Effects of Mutagenic Agents
- VI. The Nature of Somatic Cell Mutants Isolated in Culture
- References
- Chapter 8. Isolation of Metaphase Chromosomes, Mitotic Apparatus, and Nuclei
- I. Introduction
- II. General Methods
- III. Isolation Methods and Properties
- IV. Discussion
- References
- Chapter 9. Isolation of Metaphase Chromosomes with High Molecular Weight DNA at pH 10.5
- I. Introduction
- II. Cell Labeling
- III. Alkaline Sucrose Gradient Techniques
- IV. Molecular Weight Analysis of Reported Chromosome Isolation Procedures
- V. Chromosome Isolation at pH 10.5
- VI. Discussion
- References
- Chapter 10. Basic Principles of a Method of Nucleoli Isolation
- I. Introduction
- II. Determination of a Concentration Threshold of Mg Ions (Mg++) to Obtain Differential Behavior of Nucleoli and Extranucleolar Chromatin
- III. Dispersion of Extranucleolar Chromatin and Isolation of Nucleoli
- IV. Conclusion
- References
- Chapter 11. A Technique for Studying Chemotaxis of Leukocytes in Well-Defined Chemotactic Fields
- I. Introduction
- II. Methods and Materials
- III. Measurements of the Sensitivity of Polymorphonuclear Leukocytes to Cyclic Adenosine-3',5'-rnonophosphate Concentrations and Gradients
- IV. Discussion
- V. Appendix: Techniques for Evaluating the Diffusion Equations
- References
- Chapter 12. New Staining Methods for Chromosomes
- I. Introduction
- II. Preparative Techniques: A Reevaluation
- III. Principal Methods: Original and Recommended Procedures
- IV. Special Procedures and Troubleshooting
- V. Mechanisms of Banding
- VI. Significance and Applications of the New Techniques
- References
- Index
- Author Index
- Subject Index
