Methods in Cell Biology
Academic Press
Published on 31. July 1972
404 pages
978-0-08-085902-6 (ISBN)
Description
Methods in Cell Biology
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D.Marshall Prescott
Methods in Cell Physiology: v. 5
Book
07/1972
Academic Press
€136.40
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Content
- Cover
- Contents
- List of Contributors
- Preface
- Contents of Previous Volumes
- Chapter 1. Procedures for Mammalian Chromosome Preparations
- I. Introduction
- II. Animal Materials
- III. Tissue and Cell Cultures
- IV. Cytological Preparations
- Chapter 2. Cloning of Mammalian Cells
- I. Introduction
- II. Theoretical Considerations
- III. Procedures for Establishing Clonal Populations
- Appendix A. Preparation of Medium F12M
- Appendix B. Preparation of Enzyme Solutionss
- Appendix C. Cleaning of Glassware
- Appendix D. Incubation
- References
- Chapter 3. Cell Fusion and Its Application to Studies on the Regulation of the Cell Cycle
- I. Introduction
- II. Types of Cell Fusion
- III. Viruses and Cell Fusion
- IV. Methods of Cell Fusion
- V. Preparation of Some Specialized Cells for Fusion
- VI. Methods for the Selection and Isolation of Hybrids
- VII. Preparation of Fused Cells for Microscopic Examination
- VIII. Synchronization of HeLa Cells for Fusion
- IX. Application of Cell Fusion to the Study of Events Related to Cell Cycle
- X. Conclusions
- References
- Chapter 4. Marsupial Cells in Vivo and in Vitro
- I. Introduction
- II. Karyotypes of Marsupials
- III. The Marsupial Cell Cycle
- IV. Tissue Culture of Marsupial Cells
- V. Chromosome Synthetic Activities and Cytogenetics of Karyotypically Simple Cells
- VI. Concluding Remarks
- References
- Chapter 5. Nuclear Envelope Isolation
- I. Introduction
- II. Isolation of the Nuclear Envelope from Individual Cells
- III. Isolation of the Nuclear Envelope by Cell Nuclei Fractionation
- IV. Isolation of the Nuclear Envelope and Nuclear Membranes by Disintegration of Nuclei and Gradient Centrifugation
- V. Isolation of the Nuclear Envelope by Salt Extraction at High Ionic Strength
- VI. Partial Isolation of the Nuclear Envelope by Citric Acid or Detergents
- VII. Some Properties of Isolated Nuclear Membranes
- VIII. Conclusion
- References
- Addendum
- Chapter 6. Macro- and Micro-Oxygen Electrode Techniques for Cell Measurement
- I. Introduction
- II. Types of Electrode Systems
- III. Evaluation and Calibration
- IV. Calculation of Oxygen Content
- V. Measurements of Cells and Cell Fractions
- VI. Microelectrodes
- References
- Chapter 7. Methods with Tetrahymena
- I. Introduction
- II. Media
- III. Handling the Cells
- IV. Effects of Environmental Factors
- V. Synchronization
- VI . Isolation of Organelles
- VII. Inhibitors
- VIII. Radiochemical Procedures
- IX. The Cell Cycle
- X. Special Techniques
- References
- Chapter 8. Comparison of a New Method with Usual Methods for Preparing Monolayers in Electron Microscopy Autoradiography
- I. Introduction
- II. Methods
- III. Results and Discussion
- References
- Chapter 9. Continuous Automatic Cultivation of Homocontinuous and Synchronized Microalgae
- I. Introduction
- II. Characterization of Different Types of Mass Cultures
- III. Organisms and Growth Conditions
- IV. Photoelectrically Controlled Automatic Dilution
- V. Results and Accuracy in Achieving Permanent Homocontinuous Cultures
- VI. Results and Accuracy in Achieving Permanent Synchronized Cultures
- References
- Chapter 10. Vital Staining of Plant Cells
- I. Introduction
- II. Vital Staining of the Different Components of the Plant Cell
- III. General Rules for Vital Staining
- IV. Examples for Vital Staining Experiments
- Appendix A. Characteristics of some Vital Stains
- Appendix B. Possibilities for Diagnosis of the Cell Sap Content by Vital Staining
- Appendix C. Preparation of Buffer Solutions
- References
- Chapter 11. Synchrony in Blue-Green Algae
- I. Introduction
- II. Synchrony in Anacystis
- III. Synchrony of Heterocyst Production in Nostoc
- IV. Discussion
- References
- Index
- Author Index
- Subject Index
