Methods in Virology

List of ContributorsPrefaceContents of Other VolumesChapter 1-Fusion of Cells for Virus Studies and Production of Cell Hybrids I. Introduction II. Methods III. Cell Fusion in Virology IV. Selection of Hybrid Cell Lines V. Uses of Heterokaryons and Hybrid Cell Lines ReferencesChapter 2-New Approaches to Ultracentrifugation I. Introduction II. A Solution: New Approaches III. Results IV. Discussion and Summary Acknowledgments ReferencesChapter 3-Small Circular Viral DNA: Preparation and Analysis I. Introduction II. Virus Cultivation, Concentration, and Purification III. DNA Extraction and Purification IV. Physical Analysis of DNA Components V. Biological Analysis of DNA Components Appendix Acknowledgments ReferencesChapter 4-Polyacrylamide Gel Electrophoresis of Viral RNA I. Introduction II. Apparatus III. Procedures for Preparation and Running of Gels IV. Analysis of the Distribution of RNA on Polyacrylamide Gels after Electrophoresis V. Elution of RNO from Analytical Gel Fractions VI. Large-Scale Preparative Techniques VII. Applications to Virological Problems and the Analysis of Results VIII. Conclusion Acknowledgments ReferencesChapter 5-Polyacrylamide Gel Electrophoresis of Viral Proteins I. Introduction II. Procedures for Acrylamide Gel Electrophoresis III. Interpretation of Results IV. Discussion V. Appendix: Common Complaints, Troubleshooting Suggestions, and Tips ReferencesChapter 6-DNA-RNA and DNA-DNA Hybridization in Virus Research I. Introduction II. Materials III. DNA-RNA Hybridization Procedure IV. Characteristics of the DNA-RNA Hybridization Reaction V. Thermal Elution of RNA from RNA-DNA Hybrids VI. Hybridization and Elution Procedure that Minimizes RNA Degradation VII. DNA-DNO Hybridization VIII. Alternative Methods of Hybridization Acknowledgment ReferencesChapter 7-Techniques of RNA-DNA Hybridization in Solution for the Study of Viral Transcription I. Introduction II. Basic Equipment III. Preparation of Nucleic Acids IV. RNA-DNA Hybridization in Solution (Liquid Hybridization) V. Segmental Transcription Studies by RNA-DNA Hybridization in Solution ReferencesChapter 8-Methods for the Study of mRNA Synthesis in Bacteriophage-Infected E. coli I. General Introduction II. Bacteriophage-Host System III. Isolation Methods IV. Analytical Methods Acknowledgments ReferencesChapter 9-Reactions of Viruses in Agar Gel I. Introduction II. Reactions Between Viral Antigens and Antibiotics III. Reactions Between Viruses and Erythrocyte Stromata IV. Electron Microscopic Examination of Reaction Lines Acknowledgments ReferencesChapter 10-Methods for the Study of Virus-Antibody Complexes I. General Introduction II. Methods of Reactivating Neutralized Virus III. Characterization of the Virus-Antibody Complex IV. Methods of Separating the Components of a Virus-Antibody Reaction Mixture ReferencesChapter 11-Techniques of Ferritin-Tagged Antibodies I. Introduction II. Purification of Ferritin III. Preparation of Antibody Globulin Fraction from Serum IV. Conjugation of Ferritin and Antibody V. Evaluating the Conjugate VI. Methods of Direct Ferritin-Tagging VII. Methods of Indirect Ferritin-Tagging VIII. Controls in Ferritin-Tagging Experiments IX. Hybrid Antibody Method for Ferritin Localization of Antigen X. Application of Hybrid Antibody XI. Other Methods of Visualizing Immune Globulins XII. Conclusions ReferencesChapter 12-The Immunoperoxidase Technique: Localization of Viral Antigens in Cells I. Introduction II. The Principles of the Immunoperoxidase Technique III. Properties of Horseradish Peroxidase IV. Histochemical Detection of Peroxidase Activity V.