Analysing Gene Expression
A Handbook of Methods. Possibilities and Pittfalls
1st Edition
Published on 6. March 2006
XXXVIII, 954 pages
978-3-527-60533-0 (ISBN)
Description
This book combines the experience of 225 experts on 900 pages.
Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.
Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.
Reviews / Votes
"The editors...have truly provided an exception information repository on an enormous array of genetic expression-related informaion...there is no other source that provides the reader with such a clear understanding of the possibilities and pitfalls associated with each technique...highly recommended."SIM News
"The books is useful as starting points for novices, or a convenient summary of the field for those familiar with this area. The material that is covered is timely and reasonable in scope...this book is a good "one-stop-shopping" summary of this rapidly evolving area."
Cassandra Smith, Boston University, Boston, MA 02215
Biomolecular Engineering
"This two-volume book written by a total of 225 authors presents an excellent overview and deep insight into the field of eukariotic gene expression and its analysis."
ChemBioChem
Wolfgang Schumann, Universität Bayreuth
"Taken all over, this book has the potential to become the encyclopedia of gene expression analysis."
Sabine Kleinsteuber
Engineering in Life Sciences 2/2004
"...the text is clear and the illustrations are excellent. ... The work will be useful for reference, especially to provide a quick and clear summary of the various techniques for postgraduates, postdocs and older research supervisors."
Simon Baumberg, University of Leeds, Microbiology Today, Vol. 31, November 2004
More details
Other editions
Additional editions
Software
05/2004
Wiley-VCH
€429.57
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Stefan Lorkowski | Paul M. Cullen
Analysing Gene Expression
A Handbook of Methods - Possibilities and Pitfalls
Book
01/2003
1st Edition
Wiley-VCH
€312.00
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Persons
Stefan Lorkowski is the editor of Analysing Gene Expression: A Handbook of Methods, Possibilities, and Pitfalls, published by Wiley.
Paul M. Cullen is the editor of Analysing Gene Expression: A Handbook of Methods, Possibilities, and Pitfalls, published by Wiley.
Content
Preface
1 Basic concepts of gene expression
2 Sample preparation and supplementary tools
3 Standard methods for analysing mRNA expression
4 High-throughput methods of mRNA expression analysis
5 Protein expression analysis
6 Methods for mRNA and protein expression analysis in situ and in vivo
7 Bioinformatic tools and computational methods
Abbreviations
Index
List of the complete postal addresses of all authors (incl. telefone and fax numbers as well as email addresses)
1 Basic concepts of gene expression
2 Sample preparation and supplementary tools
3 Standard methods for analysing mRNA expression
4 High-throughput methods of mRNA expression analysis
5 Protein expression analysis
6 Methods for mRNA and protein expression analysis in situ and in vivo
7 Bioinformatic tools and computational methods
Abbreviations
Index
List of the complete postal addresses of all authors (incl. telefone and fax numbers as well as email addresses)
