
Chimeric RNA
Description
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Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.
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Content
Prediction, Characterization, and In Silico Validation of Chimeric RNAs.- Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope.- Identification of Chimeric RNAs Using RNA-Seq Reads and Protein-Protein Interactions of Translated Chimeras.- Gene Fusion Discovery with INTEGRATE.- Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit.- Detection and Measurement of Chimeric RNAs by RT-PCR.- Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells.- RNase Protection Assay.- Validation of Chimeric Fusion Peptides Using Proteomics Data.- NanoString nCounter Technology: High-Throughput RNA Validation.- Knockdown of Chimeric RNA by RNAi.- Overexpression of Chimeric RNA by Retroviral Transduction.- Separation of Nuclear and Cytoplasmic Fractions for Chimeric RNA Characterization.- Confirmation of Transcriptional Read-Through Events byRT-PCR.- Validating Gene Fusion as the Source of Chimeric RNAs.- Chimeric RNA and Exosomes-Based Liquid Biopsy.- SMaRT for Therapeutic Purposes.- Case Study: Landscape of Chimeric RNAs in Bladder Cancer.- Case Study: The Recurrent Fusion RNA DUS4L-BCAP29 in Non-Cancer Human Tissues and Cells.
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