Laser Capture in Microscopy and Microdissection
Academic Press
Published on 1. November 2002
775 pages
978-0-08-049701-3 (ISBN)
Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
- Basic Principles, Specialized Uses, and Genetic Applications
- LCM and its application in genomics and proteomics
- Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections
- Noncontact laser catapulting for the functional genomics and proteomics
- Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more
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11/2002
Academic Press
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Content
Comparison of Current EquipmentLaser Capture Microdissection (LCMD) and its Applications in Genomics & ProteomicsGoing in vivo With Laser MD (LMD)Use of LCMD to Selectively Obtain Distinct Populations of Cells for Proteomic AnalysisOptimized Tissue Processing and Staining for LCMD and Nucleic Acid RetrievalFluoroscence in situ Hybridization of LCM-isolated Nuclei from Paraffin SectionsImmunoblotting of Single Cell Types Isolated from Frozen Sections by LMDNoncontact Laser Catapulting: A Basic Procedure for Functl' Genomics and ProteomicsInternal Standards for LMDMethacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded TissuesUse of LCMD for Clonal AnalysisLCMD in Carcinoma AnalysisLCMD to Assess DevtApplication of LCMD to ProteomicsLCMD of the Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified.LCMD in PathologyUse of Laser Capture Microscopy (LCM) in the Analysis of Mouse Models of Human DiseasesUse of LMD in Complex TissueAssessmt of Clonal Relationships in Malignant MelanomasComp. of Normal and Tumor Cells by LCMDAnalysis of Folliculostellate Cells by LCMD and Reverse Transcription-Polymerase Chain Reaction (LCM-RT/PCR)Analysis of Gene ExpressionAnalysis of Specific Gene ExpressionGene Discovery With LCMDNA Fingerprinting from Cells Captured by LMDSingle Cell PCR in LCMAssessmt of Genetic Clonality in Tumors using LCMDGene Mutations: Analysis in Proliferative Prostatic Diseases Using LCMDUse of LCMD Generated Targets for Hybridization of High-Density Oligonucleotide Arrays. Single Cell Gene Mutation Analysis Using Laser-Assisted MD of Tissue SectionsMethylation in Gene Promoters: Assessmt after LCMD
