Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration
Published on 4. May 2012
290 pages
978-0-470-63507-0 (ISBN)
Description
A much-needed primer on the use of laser flow cytometry for stemcell analysis
Laser flow cytometry is a powerful tool for rapid analysis ofcells for marker expression, cell cycle position, proliferation,and apoptosis. However, no resources specifically address the useof this methodology for the study of stem cells; this is especiallyimportant as stem cell analysis involves specialized methods andstaining procedures based on specific characteristics such asmarker expression, cell size, drug transport, and efflux of thestem cells.
Now, this book reviews these procedures, discusses the sciencebehind them, and provides real-world examples to illustrate theusefulness of the methods. It brings together world-class expertsin pathology, biophysics, immunology, and stem cell research, whodraw upon their extensive experience with the methods and showexamples of good data to help guide researchers in the rightdirection.
Chapter coverage includes:
* Stem cell analysis and sorting using side population
* Flow cytometry in the study of proliferation and apoptosis
* Stem cell biology and application
* Identification and isolation of very small embryonic-like stemcells from murine and human specimens
* Hematopoietic stem cells--issues in enumeration
* Human embryonic stem cells: long-term culture andcardiovascular differentiation
* Limbal stem cells and corneal regeneration
* Flow cytometric sorting of spermatogonial stem cells
* Breast cancer stem cells
* Stem cell marker expression in cells from body cavityfluids
This book is an essential resource for all graduate students,practitioners in developing countries, libraries and bookrepositories of universities and research institutions, andindividual researchers. It is also of interest to laboratoriesengaged in stem cell research and use of stem cells for tissueregeneration, and to any organization dealing in stem cell andtissue regeneration research.
Reviews / Votes
"Contributors from a wide range of medical and biological sciencesexplain methods and procedures of flow cytometry specific to itsuse in research on stem cells and related matters." (SciTechBook News, December 2010)More details
Other editions
Additional editions
Awtar Krishan | H. Krishnamurthy | Satish Totey
Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration
Software
07/2012
Wiley
€137.68
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Awtar Krishan | H. Krishnamurthy | Satish Totey
Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration
Book
09/2010
1st Edition
Wiley
€170.50
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Persons
Awtar Krishan, Ph.D is a Professor of Pathology at the University of Miami School of Medicine. From 1965 to 1977, he was at the Sidney Farber Cancer Institute, Harvard MedicalSchool as Head of the Tissue Ultrastructure and Experimental Pathology Labs. Some of his recent work has focused on development and applications of a new flow cytometer which can measure cell volume and marker expression of stem cells in human apheresis samples. Dr. Krishan has more than 160 publications in peer reviewed journals.
H. Krishnamurthy is working as Scientific Officer 'E' and in charge of Flow Cytometry and Confocal Microscopy Facilities at the National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. His research interest is hormonal regulation of spermatogenesis and trafficking and signaling of gonadotropin receptors.
Satish Totey is Chief Scientific Officer, Stempeutics Research Pvt. Ltd and Director at the Manipal Institute of Regenerative Medicine inBangalore. Dr. Totey was amongst the small number of researchers who pioneered the isolation and characterization of pluripotent stem cells from human embryos. The cell lines he established have been listed in the NIH stem cell registry. He has also developed up-scaling of mesenchymal stem cells and is currently conducting phase-I and II clinical trials for myocardial infarction, leg ischemia, cerebral stroke and Multiple Sclerosis. He has published 90 research papers and holds 12US patents.
Content
CONTRIBUTORS.
PREFACE.
1 Basics of Flow Cytometry (H. Krishnamurthy and L. ScottCram).
2 Practical Considerations for Flow Cytometric Sorting of StemCells (Geoffrey W. Osborne).
3 Stem Cell Analysis and Sorting Using Side Population Analysis(William Telford).
4 Flow Cytometry in the Study of Proliferation and Apoptosis(Michael G. Ormerod and Ronald M. Hamelik).
5 Flow Cytometric Analysis of Drug Transport and Efflux in StemCells (Awtar Krishan and Ronald M. Hamelik).
6 Stem Cell Biology and Application (Swapnil Totey, RajarshiPal, Murali Krishna Mamidi, Vijayendran Govindasamy, and SatishTotey).
7 Identification and Isolation of Very Small Embryonic-like StemCells from Murine and Human Specimens (Ewa K. Zuba-Surma,Dong-Myung Shin, Izabella Klich, Janina Ratajczak, Magda Kucia, andMariusz Z. Ratajczak).
8 Electronic Volume of Hematopoietic Stem Cells (SiddharthSharma and Awtar Krishan0.
9 Hematopoietic Stem Cells: Issues in Enumeration (MichaelKeeney and D. Robert Sutherland).
10 Embryonic Stem Cells: Development and Characterization(Vijay Bhaskar R. Konala, Villoo Morawala-Patell, and AparnaKhanna).
11 Human Embryonic Stem Cells: Long-Term Culture andCardiovascular Differentiation (Maneesha Inamdar).
12 Mesenchymal Stromal Cells and Their Clinical Applications(Jyoti Kode and Vivek Tanavde).
13 Circulating Adult Stem Cells of Hematopoietic Origin forVascular and Neural Regeneration (Lissy K. Krishnan).
14 Flow Cytometric Characterization of Neural ProgenitorsDerived from Human Pluripotent Stem Cells (Raj R. Rao, Sujoy K.Dhara, Shilpa Iyer, and David W. Machacek).
15 Limbal Stem Cells and Corneal Regeneration (Geeta K.Vemuganti, Murali Mohan Sagar Balla, and Shubha Tiwari).
16 Flow Cytometric Sorting of Spermatogonial Stem Cells (B.S. Srinag, J. M. Kalappurakkal, G.H. Mohan, and H.Krishnamurthy).
17 Breast Cancer Stem Cells (Devaveena Dey and AnnapoorniRangarajan).
18 Tumor Stem Cell Marker Expression in Cells from Body CavityFluids (Awtar Krishan, Deepti Sharma, and Ronald M.Hamelik).
INDEX.
