Steroid-Cell Interactions

Introduction1 Physico-Chemical Considerations of Steroid-Receptor Interactions Steroid Structure Reactant Groups Conformation Protein Structure Quaternary Structure Secondary and Tertiary Structure Primary Structure Nucleic Acids Chemical Bonds Covalent Bond Ionic Bond Hydrogen Bond Van der Waals' Forces Hydrophobic Bond General Comments Equilibrium Constant and Free Energy Measurement of Binding Constants Reaction Kinetics2 Methods Used to Study Steroid-Tissue Receptor Interaction Cell Fractionation Cell Type Mechanical Methods Cell Culture The Use of Tumors Autoradiography Distinction between High and Low Affinity Binding Sites3 The Importance of the Metabolism of Steroid Hormones Relative to Their Binding and Mechanism of Action The Absence of the Metabolism of Steroid Hormones During Their Uptake and Retention in Many Target Cells The Metabolism of Testosterone in Androgen-Dependent Tissues The Metabolism of Progesterone in Certain Target Tissues4 Androgens and Particularly, A Metabolite of Testosterone, 5a-Dihydrotestosterone Blood Blood Cells Plasma The Distribution of NADPH-Dependent 5a-Reductase and Receptors for 5a-Dihydrotestosterone in Androgen-Sensitive Tissues and Cells The Uptake of Testosterone into Androgen-Sensitive and Androgen-Dependent Tissues The Specific, High Affinity Binding of 5a-Dihydrotestosterone in the Rat Ventral Prostate Gland The Nuclear Binding of 5a-Dihydrotestosterone Cytoplasmic Binding of 5a-Dihydrotestosterone Development of Reconstituted Systems for the Transfer of 5a-Dihydrotestosterone into Nuclei or Chromatin, In Vitro Binding of Other Steroids in the Prostate Gland Binding of Testosterone Metabolites to Prostate Microsomes Summary Binding of 5a-Dihydrotestosterone in Accessory Sexual Glands Other than Rat Ventral Prostate Gland Experimental Animals Human Prostate Gland Searches for Receptors in Other Androgen-Sensitive Systems Liver Muscle Mouse Kidney Experimental Tumors Testis Binding of Androgens in Other Tissues and Cells Female Accessory Sexual Glands Brain Pseudomonas testosteroni and Other Bacteria General Summary5 Glucocorticoids Blood Blood Cells Plasma Specific Glucocorticoid Receptor Proteins Liver Uptake of Glucocorticoids into Rat Liver The Identification of Glucocorticoid-Protein Complexes in the Cytoplasm of Rat Liver Investigations on Steroid-Protein Complexes in Rat Liver Nuclei Summary HTC Cells Uptake of Dexamethasone into HTC Cells Nuclear Binding of Glucocorticoids Binding of Glucocorticoids in the Cytoplasm Interrelationship between the Cytoplasmic and Nuclear Receptors Summary HeLa Cells The Binding of Glucocorticoids in Intact HeLa Cells The Subcellular Distribution of Glucocorticoid Binding Sites Summary Lymphocytes The Uptake of Glucocorticoids into Lymphocytes Nuclear Receptor for Glucocorticoids Cytoplasmic Binding of Glucocorticoids The Probable Conversion of the Cytoplasmic Receptor into the Nuclear Receptor The Generality of the High Affinity Glucocorticoid-Binding Process in Lymphocytes Summary Lymphoid Tumors P1798 Tumors Lymphomas Summary Fibroblasts The Uptake of Glucocorticoids into Fibroblasts Characterization of the Cytoplasmic Binding Component The Receptor Content of Glucocorticoid-Resistant L929 Cells Summary Other Tissues Mammary Gland Brain Gene