Programmed Cell Death Part B
Published on 29. July 2008
504 pages
978-0-08-088813-2 (ISBN)
Description
One of the major goals of researchers in the field of apoptosis is to identify targets for novel therapies in cancer, AIDS, and Alzheimer's disease. Understanding the molecular mechanisms of the various components of the apoptotic pathways is the first step to reaching this goal. The 2002 Nobel Prize in Physiology or Medicine was awarded to Sydney Brenner (United Kingdom), H. Robert Horvitz (US) and John E. Sulston (UK) "for their discoveries concerning genetic regulation of organ development and programmed cell death." Cell death is a fundamental aspect of embryonic development, normal cellular turnover and maintenance of homeostasis (maintaining a stable, constant environment) on the one hand, and aging and disease on the other. This volume addresses the significant advances with the techniques that are being used to analyze cell death.
- This volume provides the necessary, trusted methods to carry out this research on these latest therapeutic techniques. Once researchers understand the molecular mechanisms of the apoptotic pathways, they can begin to develop new therapies
- Presents key methods on studying tumors and how these cancer cells evade cell death
- Eliminates searching through many different sources to avoid pitfalls so the same mistakes are not made over and over
More details
Other editions
Additional editions
Roya Khosravi-Far | Zahra Zakeri | Richard A. Lockshin
Programmed Cell Death Part B: Volume 446
Book
09/2008
Academic Press
€167.13
Shipment within 15-20 days
Content
- Cover
- Copyright Page
- TOC$Contents
- Contributors
- Preface
- Volume in Series
- CH$Chapter 1: Analysis of Autophagic and Necrotic Cell Death in Dictyostelium
- 1. Introduction
- 2. Growing Dictyostelium Cells in Monolayer Cultures
- 3. Inducing Autophagic Vacuolar Cell Death
- 4. Further Exploration of Autophagic Cell Death
- 5. Inducing Necrotic Cell Death
- 6. Further Exploration of Necrotic Cell Death
- References
- CH$Chapter 2: Methods and Protocols for Studying Cell Death in Drosophila
- 1. Introduction
- 2. Cell Death Analysis During Development
- 3. Biochemical Analysis
- 4. Genetic Analysis
- References
- CH$Chapter 3: In Vivo and In Vitro Methods for Studying Apoptotic Cell Engulfment in Drosophila
- 1. Introduction
- 2. Culture Conditions
- 3. Labeling of Apoptotic Cells
- 4. Visualizing the Macrophages
- 5. Quantification and Statistics
- 6. Engulfment in Cell Culture
- Acknowledgments
- References
- CH$Chapter 4: A Mouse Mammary Epithelial Cell Model to Identify Molecular Mechanisms Regulating Breast Cancer Progression
- 1. Introduction
- 2. A Novel Mouse Mammary Epithelial Cell Model
- 3. Protocols
- 4. Concluding Remarks
- References
- CH$Chapter 5: Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer
- 1. Introduction
- 2. Immortalization of Baby Mouse Kidney, Mammary, Prostate, and Ovarian Surface Epithelial Cells
- 3. Development of iBMK Cell Lines for Identifying the Role of Apoptosis in Cancer
- 4. Aposcreen: An iBMK Cell-Based Screen for the Identification of Apoptosis-Inducing Compounds as Potential Anti-Cancer Agents
- 5. Adaptation of the iBMK Cell Model System for the Evaluation of Tissue-Specific Tumor-Promoting Functions
- 6. Tumorigenicity Assay
- 7. Noninvasive In Vivo Monitoring of Tumor Growth by Expression of Fluorescence and Chemiluminescence Markers
- 8. Tumor-Derived Cell Lines (TDCLs) as a Model System for Epithelial Tumor-Promoting Functions
- 9. Concluding Remarks and Future Perspectives
- Acknowledgments
- References
- CH$Chapter 6: DNA Damage Response and Apoptosis
- 1. Introduction
- 2. Methods
- Notes
- Acknowledgments
- References
- CH$Chapter 7: Phorbol Ester-Induced Apoptosis and Senescence in Cancer Cell Models
- 1. Introduction
- 2. PKCdelta as a Pro-Apoptotic Kinase
- 3. PKCdelta as a Mediator of Apoptotic Responses in Prostate Cancer Cells: The Emergence of an Autocrine Paradigm
- 4. Roles of PKCalpha and PKCepsilon LNCAP Cell Death and Survival
- 5. Regulation of Cell Cycle and Senescence by PKC
- 6. Determination of Phorbol Ester-Induced Apoptosis in Prostate Cancer Cells
- 7. Adenoviral Expression of PKC Isozymes
- 8. RNA Interference of PKC Isozymes
- 9. Collection and Storage of CM from LNCaP Cells
- 10. Determination of Senescence in Response to Phorbol Esters
- References
- CH$Chapter 8: Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs
- 1. Introduction
- 2. Methods
- Acknowledgments
- References
- CH$Chapter 9: Studying p53-Dependent Cell Death In Vitro and In Vivo
- 1. Introduction
- 2. Studying p53-Dependent Cell Death In Vitro
- 3. Overexpression of p53 by Adenovirus (Ad)
- 4. Silencing the p53 Gene by Retroviral Vector
- 5. Studying the Apoptotic Response
- 6. Studying p53-Dependent Apoptosis In Vivo
- References
- CH$Chapter 10: NF-kappaB as a Determinant of Distinct Cell Death Pathways
- 1. Introduction
- 2. Methods
- 3. DNA
- 4. Proteins
- 5. Metabolic Health
- 6. Senescence Detection
- References
- CH$Chapter 11: Purification and Bioassay of Hedgehog Ligands for the Study of Cell Death and Survival
- 1. Introduction
- 2. Hedgehog and the Regulation of Cell Death
- 3. Generation of Recombinant Hedgehog Ligands
- References
- CH$Chapter 12: Experimental Approaches to Investigate the Proteasomal Degradation Pathways Involved in Regulation of Apoptosis
- 1. Introduction
- 2. General Instructions
- 3. Protocols
- References
- CH$Chapter 13: Ubiquitination Mediated by Inhibitor of Apoptosis Proteins
- 1. Introduction
- 2. In Vitro Ubiquitination Assay
- 3. In Vivo Ubiquitination Assay
- Acknowledgments
- References
- CH$Chapter 14: Phosphatases and Regulation of Cell Death
- 1. Introduction
- 2. Identification of the Putative Phosphatase in Cells
- 3. In Vitro Phosphatase Assays
- 4. Interaction of the Phosphatase and Apoptotic Protein
- 5. Linking Phosphatase Activity to Cell Death Protein Function
- 6. Conclusion
- Acknowledgments
- References
- CH$Chapter 15: Analysis of Neuronal Cell Death in Mammals
- 1. Introduction
- 2. Apoptosis Detection In Vitro
- 3. Assessment of Cell Death
- 4. Apoptosis Detection in Brain Development
- 5. Detection of Synaptic Apoptosis (Synaptosis)
- 6. Conclusions
- Acknowledgments
- References
- CH$Chapter 16: Dissecting Apoptosis and Intrinsic Death Pathways in the Heart
- 1. Introduction
- 2. Protocols
- Acknowledgments
- References
- CH$Chapter 17: Quantification of Vascular Endothelial Cell Apoptosis In Vivo
- 1. Introduction
- 2. Protocols
- References
- CH$Chapter 18: Recombinant TRAIL and TRAIL Receptor Analysis
- 1. Introduction
- 2. Generation of Recombinant TRAIL
- 3. Analysis of TRAIL Receptors
- 4. Concluding Remarks
- Acknowledgments
- References
- CH$Chapter 19: Analysis of Tnf-Related Apoptosis-Inducing Ligand In Vivo Through Bone Marrow Transduction and Transplantation
- 1. Introduction
- 2. Generation of Retroviral Infection Medium
- 3. Bone Marrow Cell Preparation
- 4. Retroviral Transduction of Bone Marrow Cells
- 5. Bone Marrow Transplantation
- 6. Assessment of Bone Marrow Transplantation
- 7. Pathologic Evaluation of TRAIL-Transduced Mice
- 8. Methods for Immunohistochemical Staining for TRAIL Expression
- 9. Applications that Use the Bone Marrow Transplantation Approach
- Acknowledgments
- References
- CH$Chapter 20: Overcoming Resistance to Trail-Induced Apoptosis in Prostate Cancer by Regulation of c-FLIP
- 1. Introduction
- 2. Structure and Molecular Mechanisms of c-FLIP in Apoptosis
- 3. Expression of c-FLIP
- 4. Regulation of c-FLIP Expression
- 5. Targeting c-FLIP to Enhance Apoptosis in Cancer Cells
- 6. Methods and Materials
- Acknowledgments
- References
- CH$Chapter 21: Caspase Assays: Identifying Caspase Activity and Substrates In Vitro and In Vivo
- 1. Introduction
- 2. Caspase Methods
- 3. Conclusion
- References
- CH$Chapter 22: Synthesis and Biophysical Characterization of Stabilized alpha-Helices of BCL-2 Domains
- 1. Introduction
- 2. Synthesis of Non-Natural Amino Acids for Peptide Stapling
- 3. Design and Synthesis of Stabilized alpha-Helices of BCL-2 Domains (SAHBs)
- 4. Structural Assessment of SAHBs by Circular Dichroism (CD)
- 5. Protease Resistance Testing of SAHBs
- 6. Cell Permeability Screening of SAHBs
- 7. Summary
- Acknowledgments
- References
- CH$Chapter 23: Dissection of the BCL-2 Family Signaling Network with Stabilized alpha-Helices of BCL-2 Domains
- 1. Introduction
- 2. BCL-2 Family Binding Measurements by Fluorescence Polarization Assay
- 3. In Vitro Release Assays as a Measure of Pro-Apoptotic Activity
- 4. Measurement of Cellular Apoptosis Induction
- 5. Identification of In Situ Mechanistic Targets of SAHBs
- 6. Summary
- Acknowledgments
- References
- CH$Chapter 24: ERM-Mediated Genetic Screens in Mammalian Cells
- 1. Introduction
- 2. Methods and Design
- 3. ERM-Mediated Genetic Screens and Re-PCA
- 4. Conclusions
- Acknowledgments
- References
- Author Index
- IDX$Subject Index
- Color Plate Section
