Nonradioactive Labeling and Detection of Biomolecules

1 General Aspects of Nonradioactive Labeling and Detection.- 1.1 Introduction.- 1.2 The Concept of Nonradioactive Bioanalytics..- 1.3 Direct and Indirect Labeling and Detection Systems.- 1.4 Labeling Methods.- 1.5 Detection Methods.- 1.6 Guide of the Use of Information in this Book.- I Standard Nonradioactive Labeling and Detection Systems.- 2 Overview of Nonradioactive Labeling Systems.- 3 The Digoxigenin: Anti-Digoxigenin (DIG) System.- 4 The Biotin System.- 5 In Vivo Labeling of DNA Probes with 5-BrdU.- 6 The Sulfone System.- 7 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Probes.- 8 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection.- 9 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays.- 10 The SNAP System.- II Specialized Nonradioactive Detection Systems.- 11 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems.- 12 Colorimetric Systems.- 13 Luminescence Systems.- 14 Fluorescence Systems.- III Enhanced Systems.- 15 Overview of Amplification Systems.- 16 Enhanced Signal Generation by Target Amplification.- 17 Signal Amplification System: Substrate Cascade.- IV Application Formats.- 18 Overview of Application Formats.- 19 Blot Formats: Nucleic Acids.- 20 Blot Formats: Proteins and Glycoproteins.- 21 In Situ Formats.- 22 Quantitative Formats.- Appendix I Suppliers of Reagents.- Appendix II Suppliers of Equipment.