
ELISA: History, Types and Applications
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Content
- Intro
- Contents
- Preface
- Acknowledgments
- Acronyms
- Chapter 1
- Chapter 2
- Chapter 3
- Chapter 4
- Chapter 5
- Chapter 6
- Chapter 7
- Chapter 8
- Chapter 1
- The History and Background of Enzyme-Linked Immunosorbent Assay
- Abstract
- Introduction
- Methods
- History and Background
- Coating/Capture
- Plate Blocking
- Probing/Detection
- Signal Measurement
- Immunoassay
- Competitive and Noncompetitive
- Homogenous and Heterogeneous
- Important Components in Immunoassay
- Antibody (Antiserum)
- Antigen and Hapten
- Polyclonal Antibody and Monoclonal Antibody
- Typical Steps of an ELISA
- Indirect Detection
- Signal Output and Assay Performance
- Factors Affecting Assembly of the Immune Complex
- Types of Plate and Coupling Options
- Antibodies
- Blocking Buffer
- Target Antigen
- Enzyme Conjugate
- Washing the Plate
- Substrates and Signal Detection
- Quantitative
- Qualitative
- Semi-Quantitative
- References
- Chapter 2
- Types of Enzyme-Linked Immunosorbent Assays
- Abstract
- 1. Introduction
- 2. Methods
- 2.1. Classification
- 2.1.1. Classification According to the Solid-Phase Carrier Material
- 2.1.1.1. Microplate ELISA
- 2.1.1.2. Double Antibody Sandwich Method
- 2.1.1.3. Two-Site One-Step Method
- 2.1.1.4. Double Sandwich ELISA
- 2.1.1.5. Double-Antigen Sandwich Method
- 2.1.1.6. Competition ELISA
- 2.1.1.7. Capture Method
- 2.1.1.8. Blocking Method
- 2.2. Dot-ELISA
- 2.3. Cloth-ELISA (C-ELISA)
- 3. Classification Based on Reaction Process
- 3.1. The Direct Method
- 3.2. Indirect Method
- 4. ELISA Coupled with Other Techniques
- 4.1. ELISA Using Avidin and Biotin
- 4.2. PCR-ELISA
- 4.3. Monoclonal Antibody Capture ELISA
- References
- Chapter 3
- ELISA: From Theory to Practice
- Abstract
- Introduction
- Methods
- Theory to Practice
- Choosing an ELISA Type
- Direct ELISA
- Indirect ELISA
- Sandwich ELISA
- Competitive ELISA
- ELISA Components and Considerations
- Plates
- Buffers
- Coating Buffer
- Washing Buffer
- Blocking Buffer
- Antibodies
- Antibody-Antigen Complexes
- Monoclonal Antibodies and Matched Pairs
- Polyclonal Antibodies
- Sampling, Handling, Preparation
- Dilution
- Washing
- Enzyme-Substrate Combinations
- ELISA Optimisation
- Coating Buffer
- Blocking Buffer
- Sample Concentration
- Detection Antibody
- Enzyme Conjugate
- Troubleshooting
- References
- Chapter 4
- Application of Enzyme-Linked Immunosorbent Assay
- Abstract
- Introduction
- Methods
- Application
- ELISA and Clinical Medicine
- ELISA Detection of Antigens and Haptens
- ELISA Detection Antibody
- ELISA and Veterinary Medicine
- ELISA and Animal Epidemic Disease Detection
- ELISA and Veterinary Drug Residue Detection
- Tetracycline Drug Detection
- Chloramphenicol Drug Detection
- Nitrofurans Drug Detection
- Detection of Sulfonamides
- Hormone Drug Detection
- ELISA and Plant Resources
- ELISA and Food Safety
- Detecting Pesticide Residues in Food
- Detection of Pathogenic Microorganisms in Food
- Detecting Biological Toxins in Food
- Detecting Illegally Added Substances in Food
- Detection of Transgenic Foods
- Detection of Allergens
- ELISA and Basic Medicine
- ELISA and Environmental Testing
- References
- Chapter 5
- Enzyme-Linked Immunoassay, Advanced Glycation End Products, and their Cell Surface Receptor in Metabolic Diseases
- Abstract
- Introduction
- Methods
- Enzyme-Linked Immunoassay in an AGE/RAGE Axis
- AGEs and Their Formation
- Dietary AGEs
- AGE Damage Mechanism
- Receptor-Independent Mechanism
- Receptor-Dependent Damage
- ELISA as the Main Technique for AGE Assay in Clinical Research
- dAGE Measurement
- AGE Role in Metabolic Diseases
- Healthy Subjects
- Cross-Sectional Studies
- Clinical Trials
- Renal Disease
- Diabetes Mellitus
- Subjects with Obesity and Cardiometabolic Risk
- Conclusion
- References
- Chapter 6
- The Development of an Indirect ELISA Test for the Diagnosis of Brucella canis
- Abstract
- Introduction
- Methods
- Development of Indirect-ELISA test
- Preparation of Antigen
- Indirect Enzyme-Linked Immunosorbent Assay (I-ELISA)
- Determination of Cut-Off Value
- Blood Serum Sample
- Testing of Developed I-ELISA Test
- References
- Chapter 7
- The Key Role of Immunoassay Based Detection in Cancer Research
- Abstract
- Introduction
- Methods
- Immunoassay Based Detection in Cancer Research
- Immunomolecular Markers for Hematologic Malignancies
- Acute Lymphoblastic Leukemia
- Circulating Ki-67 for Prognosis
- Circulating Soluble LR11 Levels are Highly Increased in Acute Leukemias
- Acute Myeloid Leukemia
- Soluble Urokinase Plasminogen Activator Receptor (suPAR) for Diagnosis and Monitoring of Treatment in Patients with Hematologic Malignancies
- Chronic Lymphocytic Leukemia
- CD44 and IFN-?
- Progranulin
- Hodgkin Lymphoma
- CD83 As a New Potential Biomarker and Therapeutic Target for Hodgkin Lymphoma
- ARg-1
- Galectin-1, Tumor Burden and Adverse Clinical Features
- Non-Hodgkin Lymphoma
- Soluble Interleukin-2 Receptor Levels in Serum of Patients with Hematological or Non-Hematological Malignancies
- Multiple Myeloma
- Angiopoietin-2
- Conclusion
- Immunoassay Approaches in Solid Tissue Malignancies
- Breast Cancer
- Serum Markers
- Hepatocellular Carcinoma
- Serum Markers
- Pancreatic Cancer
- Serum Markers
- Gastric Cancer
- Serum Markers
- Esophageal Cancer
- Serum Markers
- Colorectal Cancer
- Serum Markers
- Future Trends: Mass Spectrometry and ELISA
- References
- Chapter 8
- Enzyme-Linked Immunosorbent Assay for the Detection of Helicobacter pylori
- Abstract
- Introduction
- Methods
- Detection of Helicobacter pylori
- References
- About the Editor
- Index
- Blank Page
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